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Team:BostonU/Training
From 2014.igem.org
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<tr> | <tr> | ||
| - | < | + | <td colspan="2" scope="col">This notebook details the concepts, methods, and protocols learned throughout the iGEM process by all of the BU team members.</td> |
</tr> | </tr> | ||
<br> | <br> | ||
<tr> | <tr> | ||
| - | < | + | <td colspan="2" scope="col"><br><h2>May</h2></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | < | + | <td colspan="2" scope="col"><h3>Week of May 12</h3></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | < | + | <td colspan="2" scope="col">• Learned about synthetic biology, iGEM, and MoClo. <br> |
• Became familiar with lab part and BioBrick part nomenclature.<br> | • Became familiar with lab part and BioBrick part nomenclature.<br> | ||
• Learned how to use miniEugene from CIDAR lab members.<br> | • Learned how to use miniEugene from CIDAR lab members.<br> | ||
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• Began using miniEugene to design architecture of constructs.<br> | • Began using miniEugene to design architecture of constructs.<br> | ||
• Struck out plates with 15 L0 parts, 6 L1 parts, and 2 L2 parts and grew colonies in liquid cultures overnight.<br> | • Struck out plates with 15 L0 parts, 6 L1 parts, and 2 L2 parts and grew colonies in liquid cultures overnight.<br> | ||
| - | </ | + | </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | < | + | <td colspan="2" scope="col"><h3>Week of May 19</h3></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | < | + | <td colspan="2" scope="col">• Performed minipreps and quantified the DNA concentration of parts using a Nanodrop spectrophotometer.<br> |
• Sent the parts for sequencing, all but four of which were correctly verified using BLAST. Parts not correctly verified were re-submitted for sequencing.<br> | • Sent the parts for sequencing, all but four of which were correctly verified using BLAST. Parts not correctly verified were re-submitted for sequencing.<br> | ||
| - | • Transformed verified parts into E. Coli Bioline cells and performed colony PCR and gel electrophoresis.</ | + | • Transformed verified parts into E. Coli Bioline cells and performed colony PCR and gel electrophoresis.</td> |
</tr> | </tr> | ||
| - | <tr>< | + | <tr><td colspan="2" scope="col"><h3>Week of May 26</h3></td></tr> |
| - | <tr>< | + | <tr><td colspan="2" scope="col">• Analyzed gels and picked colonies.<br> |
• Performed minipreps on gel-verified strains.<br> | • Performed minipreps on gel-verified strains.<br> | ||
• Made glycerol stocks of confirmed parts.<br> | • Made glycerol stocks of confirmed parts.<br> | ||
• Set up and performed MoClo reactions to make 2 new L1 parts.<br> | • Set up and performed MoClo reactions to make 2 new L1 parts.<br> | ||
| - | • Transformed MoClo reactions and grew up over the weekend.</ | + | • Transformed MoClo reactions and grew up over the weekend.</td> |
</tr> | </tr> | ||
<br> | <br> | ||
| - | <tr>< | + | <tr><td colspan="2" scope="col"><h2>June</h2></td></tr> |
| - | <tr>< | + | <tr><td colspan="2" scope="col"><h3>Week of June 2</h3></td></tr> |
| - | <tr>< | + | <tr><td colspan="2" scope="col">• Picked colonies, miniprepped, quantifies, and sent parts for sequence verification.<br> |
• Set up and performed MoClo reaction for 2 new L1 and 2 new L2 parts.<br> | • Set up and performed MoClo reaction for 2 new L1 and 2 new L2 parts.<br> | ||
• Transformed reactions and grew up overnight.<br> | • Transformed reactions and grew up overnight.<br> | ||
| - | • Brainstormed to further define project goals.</ | + | • Brainstormed to further define project goals.</td> |
</tr> | </tr> | ||
| - | <tr>< | + | <tr><td colspan="2" scope="col"><h3>Week of June 9</h3></td></tr> |
| - | <tr>< | + | <tr><td colspan="2" scope="col">• Participated in SB2 (Synthetic Biology Boston) and IWBDA (Internatonal Workshop on Bio-Design Automation) workshops hosted at BU.</td></tr> |
| - | <tr>< | + | <tr><td colspan="2" scope="col"><h3>Week of June 16</h3></td></tr> |
| - | <tr>< | + | <tr><td colspan="2" scope="col">• Set up parts for FACS experiment.<br> |
| - | • Participated in NEGEM meetup.</ | + | • Participated in NEGEM meetup.</td> |
</tr> | </tr> | ||
Latest revision as of 21:51, 15 October 2014
| This notebook details the concepts, methods, and protocols learned throughout the iGEM process by all of the BU team members. | |
May |
|
Week of May 12 |
|
| • Learned about synthetic biology, iGEM, and MoClo. • Became familiar with lab part and BioBrick part nomenclature. • Learned how to use miniEugene from CIDAR lab members. • Developed plan for next two weeks that would guide through training of essential lab protocols - specifically plate streaking, minipreps, MoClo, sequencing, and colony PCR. • Began using miniEugene to design architecture of constructs. • Struck out plates with 15 L0 parts, 6 L1 parts, and 2 L2 parts and grew colonies in liquid cultures overnight. |
|
Week of May 19 |
|
| • Performed minipreps and quantified the DNA concentration of parts using a Nanodrop spectrophotometer. • Sent the parts for sequencing, all but four of which were correctly verified using BLAST. Parts not correctly verified were re-submitted for sequencing. • Transformed verified parts into E. Coli Bioline cells and performed colony PCR and gel electrophoresis. |
|
Week of May 26 | |
| • Analyzed gels and picked colonies. • Performed minipreps on gel-verified strains. • Made glycerol stocks of confirmed parts. • Set up and performed MoClo reactions to make 2 new L1 parts. • Transformed MoClo reactions and grew up over the weekend. |
|
June | |
Week of June 2 | |
| • Picked colonies, miniprepped, quantifies, and sent parts for sequence verification. • Set up and performed MoClo reaction for 2 new L1 and 2 new L2 parts. • Transformed reactions and grew up overnight. • Brainstormed to further define project goals. |
|
Week of June 9 | |
| • Participated in SB2 (Synthetic Biology Boston) and IWBDA (Internatonal Workshop on Bio-Design Automation) workshops hosted at BU. | |
Week of June 16 | |
| • Set up parts for FACS experiment. • Participated in NEGEM meetup. |
