Team:BostonU/Training

From 2014.igem.org

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         <th colspan="2" scope="col">Page content</th>
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         <td colspan="2" scope="col">This notebook details the concepts, methods, and protocols learned throughout the iGEM process by all of the BU team members.</td>
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<td colspan="2" scope="col"><br><h2>May</h2></td>
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<td colspan="2" scope="col"><h3>Week of May 12</h3></td>
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        <td colspan="2" scope="col">• Learned about synthetic biology, iGEM, and MoClo. <br>
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• Became familiar with lab part and BioBrick part nomenclature.<br>
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• Learned how to use miniEugene from CIDAR lab members.<br>
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• Developed plan for next two weeks that would guide through training of essential lab protocols - specifically plate streaking, minipreps, MoClo, sequencing, and colony PCR.<br>
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• Began using miniEugene to design architecture of constructs.<br>
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• Struck out plates with 15 L0 parts, 6 L1 parts, and 2 L2 parts and grew colonies in liquid cultures overnight.<br>
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<td colspan="2" scope="col"><h3>Week of May 19</h3></td>
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<td colspan="2" scope="col">• Performed minipreps and quantified the DNA concentration of parts using a Nanodrop spectrophotometer.<br>
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• Sent the parts for sequencing, all but four of which were correctly verified using BLAST. Parts not correctly verified were re-submitted for sequencing.<br>
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• Transformed verified parts into E. Coli Bioline cells and performed colony PCR and gel electrophoresis.</td>
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<tr><td colspan="2" scope="col"><h3>Week of May 26</h3></td></tr>
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<tr><td colspan="2" scope="col">• Analyzed gels and picked colonies.<br>
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• Performed minipreps on gel-verified strains.<br>
 +
• Made glycerol stocks of confirmed parts.<br>
 +
• Set up and performed MoClo reactions to make 2 new L1 parts.<br>
 +
• Transformed MoClo reactions and grew up over the weekend.</td>
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<tr><td colspan="2" scope="col"><h2>June</h2></td></tr>
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<tr><td colspan="2" scope="col"><h3>Week of June 2</h3></td></tr>
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<tr><td colspan="2" scope="col">• Picked colonies, miniprepped, quantifies, and sent parts for sequence verification.<br>
 +
• Set up and performed MoClo reaction for 2 new L1 and 2 new L2 parts.<br>
 +
• Transformed reactions and grew up overnight.<br>
 +
• Brainstormed to further define project goals.</td>
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<tr><td colspan="2" scope="col"><h3>Week of June 9</h3></td></tr>
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<tr><td colspan="2" scope="col">• Participated in SB2 (Synthetic Biology Boston) and IWBDA (Internatonal Workshop on Bio-Design Automation) workshops hosted at BU.</td></tr>
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<tr><td colspan="2" scope="col"><h3>Week of June 16</h3></td></tr>
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<tr><td colspan="2" scope="col">• Set up parts for FACS experiment.<br>
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• Participated in NEGEM meetup.</td>
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<br><br><br><br><br><div class="sponsors" align="center"> <br><br><header1>Our Sponsors</header1><br><br><img src="https://static.igem.org/mediawiki/2014/c/c5/Sponsors_bu14.png" width="983" height="149"> </div>
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Latest revision as of 21:51, 15 October 2014



Training Notebook

This notebook details the concepts, methods, and protocols learned throughout the iGEM process by all of the BU team members.

May

Week of May 12
• Learned about synthetic biology, iGEM, and MoClo.
• Became familiar with lab part and BioBrick part nomenclature.
• Learned how to use miniEugene from CIDAR lab members.
• Developed plan for next two weeks that would guide through training of essential lab protocols - specifically plate streaking, minipreps, MoClo, sequencing, and colony PCR.
• Began using miniEugene to design architecture of constructs.
• Struck out plates with 15 L0 parts, 6 L1 parts, and 2 L2 parts and grew colonies in liquid cultures overnight.

Week of May 19
• Performed minipreps and quantified the DNA concentration of parts using a Nanodrop spectrophotometer.
• Sent the parts for sequencing, all but four of which were correctly verified using BLAST. Parts not correctly verified were re-submitted for sequencing.
• Transformed verified parts into E. Coli Bioline cells and performed colony PCR and gel electrophoresis.

Week of May 26

• Analyzed gels and picked colonies.
• Performed minipreps on gel-verified strains.
• Made glycerol stocks of confirmed parts.
• Set up and performed MoClo reactions to make 2 new L1 parts.
• Transformed MoClo reactions and grew up over the weekend.

June

Week of June 2

• Picked colonies, miniprepped, quantifies, and sent parts for sequence verification.
• Set up and performed MoClo reaction for 2 new L1 and 2 new L2 parts.
• Transformed reactions and grew up overnight.
• Brainstormed to further define project goals.

Week of June 9

• Participated in SB2 (Synthetic Biology Boston) and IWBDA (Internatonal Workshop on Bio-Design Automation) workshops hosted at BU.

Week of June 16

• Set up parts for FACS experiment.
• Participated in NEGEM meetup.







Our Sponsors

Retrieved from "http://2014.igem.org/Team:BostonU/Training"