Team:Heidelberg/testing/Parts

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<p>Promoters are the key regulators of gene expression. Possessing promoters which are active under a desired condition, at a desired strength and in a specified tissue is of great value for Plant Biotechnology, Gene Therapy and fundamental research in Bioscience. Therefore, it has become a desire to synthetically construct promoters responsive to a variety of pathways. We explore two ways to the synthesis of promoters: On one hand, we have developed a bioinformatical model and database (HEARTBEAT) describing the structure of promoters responsive to user-defined inputs. On the other hand, we have developed a biochemical method for the synthesis of randomized promoter libraries. Using this method, we have created a library of constitutive promoters of varying strength. Also, we have created libraries of promoters putatively responsive to a variety of pathways. We have screened these libraries for functional, pathway responsive promoters and present a detailed characterization of a NF-κB responsive promoter of our making. We finally discuss ways to combine randomized biochemical synthesis and bioinformatical modeling to propose a method towards the generation of promoters of complex regulation (i.e. by multiple pathways).</p>
 
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      <div class="cell" style="width:60%;">
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        <a href="#" class="box" style="margin-bottom:15px; display:block;">
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          <h3>New <span>RFC</span> iGEM_HD14</h3>
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          Hier findet der Leser eine Erklärung. Hier findet der Leser eine Erklärung.
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          Hier findet der Leser eine Erklärung. Hier findet der Leser eine Erklärung.Hier findet der Leser eine Erklärung. Hier findet der Leser eine Erklärung.
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        </a>
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        <a href="#" class="box" style="display:block;">
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          <h3>the <span>intein</span> library</h3>
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          Hier findet der Leser eine Erklärung. Hier findet der Leser eine Erklärung.
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        </a>
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      <a href="#" class="box cell" style="position:relative;">
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        <h3>Our <span>favorite</span> Parts</h3>
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        Take a look at our favorite Parts for Circularization, Fusion or Tagging and see how our system works in the living cell. For that visit our Sample Data Page.
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      <img src="/wiki/images/9/9b/Heidelberg_Favoriteparts_star.png" class="star" alt="Favorite Parts Image" />
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        <h3>Our <span>BioBricks</span></h3>
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        Hier findet der Leser eine Erklärung. Hier findet der Leser eine Erklärung. Hier findet der Leser eine Erklärung.
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      </a>
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        <h3>Our <span>Backbones</span></h3>
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        Hier findet der Leser eine Erklärung. Hier findet der Leser eine Erklärung
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        Hier findet der Leser eine Erklärung. Hier findet der Leser eine ErklärungHier findet der Leser eine Erklärung. Hier findet der Leser eine ErklärungHier findet der Leser eine Erklärung. Hier findet der Leser eine ErklärungHier findet der Leser eine Erklärung. Hier findet der Leser eine Erklärung. Hier findet der Leser eine Erklärung.
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        <h3><span>Characterization</span> of an existing Part</h3>
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        Hier findet der Leser eine Erklärung. Hier findet der Leser eine Erklärung. Hier findet der Leser eine Erklärung.
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Latest revision as of 20:46, 6 October 2014