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Team:BostonU/Workflow
From 2014.igem.org
(Difference between revisions)
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• Add parts to <a href="https://2014.igem.org/Team:BostonU/MoClo">MoClo library</a>. These parts were found to be necessary for our priority encoder:<br><br> | • Add parts to <a href="https://2014.igem.org/Team:BostonU/MoClo">MoClo library</a>. These parts were found to be necessary for our priority encoder:<br><br> | ||
| - | <p class="tab">• 3 MoClo level 1 and 3 MoClo level 2 backbones, each with a different <a href="https://2014.igem.org/Team:BostonU/Backbones">origin of replication</a>:<br> | + | <p class="tab">• 3 MoClo level 1 and 3 MoClo level 2 backbones, each with a different <a href="https://2014.igem.org/Team:BostonU/Backbones">origin of replication</a>:<br></p> |
<p class="dtab"> | <p class="dtab"> | ||
• ColE1<br> | • ColE1<br> | ||
| Line 34: | Line 34: | ||
</p> | </p> | ||
| - | • X <a href="https://2014.igem.org/Team:BostonU/FusionProteins">fusion proteins</a>:<br> | + | <p class="tab">• X <a href="https://2014.igem.org/Team:BostonU/FusionProteins">fusion proteins</a>:<br></p> |
<p class="dtab"> | <p class="dtab"> | ||
• TetR_GFP<br> | • TetR_GFP<br> | ||
| Line 40: | Line 40: | ||
</p> | </p> | ||
| - | • X <a href="https://2014.igem.org/Team:BostonU/ProjectTandemPromoters">tandem promoters</a>:<br> | + | <p class="tab">• X <a href="https://2014.igem.org/Team:BostonU/ProjectTandemPromoters">tandem promoters</a>:<br> |
<p class="dtab"> | <p class="dtab"> | ||
• pTet_pLac<br> | • pTet_pLac<br> | ||
• ...<br><br> | • ...<br><br> | ||
| - | + | </p> | |
Revision as of 17:39, 23 September 2014
Phase I - Build and test basic parts. | |
|---|---|
General Chimera Workflow |
Case Study: BU Priority Encoder |
| general I |
• Add parts to MoClo library. These parts were found to be necessary for our priority encoder: • 3 MoClo level 1 and 3 MoClo level 2 backbones, each with a different origin of replication:
• ColE1 • X fusion proteins:
• TetR_GFP • X tandem promoters:
• pTet_pLac |
