Team:BostonU/Workflow

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(Difference between revisions)
Line 28: Line 28:
<p class="tab">• 3 MoClo level 1 and 3 MoClo level 2 backbones, each with a different <a href="https://2014.igem.org/Team:BostonU/Backbones">origin of replication</a>:<br>
<p class="tab">• 3 MoClo level 1 and 3 MoClo level 2 backbones, each with a different <a href="https://2014.igem.org/Team:BostonU/Backbones">origin of replication</a>:<br>
 +
<p class="tab">
• ColE1<br>
• ColE1<br>
• p15A<br>
• p15A<br>
• pSC101<br><br>
• pSC101<br><br>
 +
</p>
• X <a href="https://2014.igem.org/Team:BostonU/FusionProteins">fusion proteins</a>:<br>
• X <a href="https://2014.igem.org/Team:BostonU/FusionProteins">fusion proteins</a>:<br>

Revision as of 17:38, 23 September 2014



Workflow

Phase I - Build and test basic parts.

General Chimera Workflow

Case Study: BU Priority Encoder

general I • Add parts to MoClo library. These parts were found to be necessary for our priority encoder:

• 3 MoClo level 1 and 3 MoClo level 2 backbones, each with a different origin of replication:

• ColE1
• p15A
• pSC101

• X fusion proteins:
• TetR_GFP
• ...

• X tandem promoters:
• pTet_pLac
• ...








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