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Team:BostonU/Workflow
From 2014.igem.org
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| - | + | • Add parts to <a href="https://2014.igem.org/Team:BostonU/MoClo">MoClo library</a>. These parts were found to be necessary for our priority encoder:<br><br> | |
| - | • 3 MoClo level 1 and 3 MoClo level 2 backbones, each with a different <a href="https://2014.igem.org/Team:BostonU/Backbones">origin of replication</a>:<br> | + |  • 3 MoClo level 1 and 3 MoClo level 2 backbones, each with a different <a href="https://2014.igem.org/Team:BostonU/Backbones">origin of replication</a>:<br> |
• ColE1<br> | • ColE1<br> | ||
• p15A<br> | • p15A<br> | ||
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• pTet_pLac<br> | • pTet_pLac<br> | ||
• ...<br><br> | • ...<br><br> | ||
| - | |||
Revision as of 17:33, 23 September 2014
Phase I - Build and test basic parts. | |
|---|---|
General Chimera Workflow |
Case Study: BU Priority Encoder |
| general I |
• Add parts to MoClo library. These parts were found to be necessary for our priority encoder: • 3 MoClo level 1 and 3 MoClo level 2 backbones, each with a different origin of replication: • ColE1 • p15A • pSC101 • X fusion proteins: • TetR_GFP • ... • X tandem promoters: • pTet_pLac • ... |
