Team:Oxford/how much can we degrade
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- | <h1blue2> | + | <h1blue2>Using buffers to reduce the pH change of our system</h1blue2> |
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+ | As one of the products of our reaction is hydrochloric acid, we have been able to calculate the pH change of the system. However, as pH change is bad for the bacteria, we have investigated the effect of using pH buffers in the aqueous part of our system. | ||
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+ | The pH change of our system in the presence of the buffer HEPES is described by: | ||
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+ | <img src="https://static.igem.org/mediawiki/2014/3/31/Oxford_DCMdeg16.png" style="float:left;position:relative; width:60%; margin-left:0%; margin-right:40%;margin-bottom:2%;" /> | ||
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+ | Upon solving the equation in Matlab, it was clear that only a relatively low concentration (0.05 M) of buffer was needed to significantly reduce the pH change of the solution: | ||
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+ | <img src="https://static.igem.org/mediawiki/2014/7/76/Oxford_DCMdeg17.png" style="float:left;position:relative; width:100%; margin-left:0%; margin-right:0%;margin-bottom:2%;" /> | ||
+ | The other method of reducing the overall pH change is adding much more water to the system at the start. This is the easiest and cheapest method, indeed it could be used for single use DCM disposal kits. However it is impractical in large scale applications due to the huge advantages of using buffer. On the other hand, because the buffer itself is toxic in high enough concentrations, a compromise between the amount of water added and buffer concentration had to be reached. | ||
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Revision as of 22:18, 21 September 2014
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