Team:Korea U Seoul/Notebook
From 2014.igem.org
Meeting
- First meeting with 2014 kuas team members. Discussed about what is kuas, synthetic biology and igem.
Information
- Synthetic biology homepage : http://syntheticbiology.org
- Part legistry page : http://part.igem.org
Homework
- investigate about subject of2008, 2009 igem teams investigate about subject of 2008, 2009 igem teams
Study of igem 2008~2009 colleges
- Outer membrane vesicles (OMV) : gram negative bacteria like E. coli have Outer membrane vesicles (OMV), which can transfer toxin to other bacteria or host strains. Omv is composed of bi-lipid layer with water soluble core, so it can transfer both water soluble materials and lipid soluble materials. Omv is a strong candidate in long-distance messaging.
- Different color expression by density
- Check the expression by density divided strong, wild, weak Lock & key, Jammer
- Formation of hybrid-promoter, light-sensing, biomaterial production
- RNA double helix come untied when increase the heat.
- using NhaA, Urea sensor
Tips of instrutor
Discussion of direction
Study presentation
- Siderosphore : Fe3+ absorb, high reducing agent. =>use ocean microbes to greenhouse effect, pathogen control, biofuel
- Degradation Aplatoxin
- GABA : neuro-material.
- T3SS(Type 3 secretion system) : related to plant immunity. Mechanism has been found.
- Pathogen microorganism sensor : use metabolic product as marker like enterotoxin or virulence factor
- PCN(antifungal product)
- Shiga toxin producing E. coli : can develop to cell to cell communication. Herein.
- checker of cosmetics: ex) PH sensor
- dye : E. coli producing indigo
- Pectinase+ beta-chrotin, E. coli producing 3-4 nutrients-> making juice
- bio-filter
- removal odors of food junks : ex)remove pathogenity.
- Soil sensor
Next week
- minseob- GABA, Shiga toxin, components of cosmetics
- jihee- effectiveness of making juice
- hyeyeong- effectiveness of removing bad smells and probability of removing pathogenity
- juyeong- about iron ion, removing ground torpedo microbes
About ideas
- extension of siderosphore idea
- papers of Sigatoxin
- papers about pection production
- papers of streptomycetes, actinomycetes
Next week
- kyungwoo- feedback to papers
- others- get feedbacks and more study about each idea.
Schedule
Fix idea until middle of January
Accounts
In |
Korean dollar |
Out |
Korean dollar |
2013 |
|
Team registration fee |
300 |
left |
100 |
Participation fee |
250 |
KOFST |
900 |
plane |
1020 |
CCP |
250 |
abode |
360 |
2014 |
|
Team tea-shirts |
30 |
CCP |
250 |
|
|
KOFST |
150 |
|
|
Self-administration of KU |
60 |
|
|
Mycroryza |
10 |
|
|
festival |
30 |
|
|
fee |
20 |
|
|
sum |
1860 |
sum |
1960 |
Presentation of ideas
- Siderophore : absorb iron ion FUR system ) Ferric Uptake Regulator.
- Aflatoxin
- Shiga toxin @ shiga toxin producing E. coli: cause hemolytic-uremic syndrome (Proprotein convertases :change proprotein to protein )
- Messages in a bubble : outer membrane vesicle (OMV)
- Collaboration : proprotein caspases (one of serine proteases, subtilisin serine proteases) , send subtilisin to STEC (destroyed shiga toxin will be sent to eukaryotic, but it won’t work. )
- Develop experiments of Paris
- pectinase production from E. coli
- Cellulase+pectinase-> more degradation of pulp
- Use residuum of fruits by pectinase
- Removing bad smells of food junks
- the main components of bad smells include nitrogen->regulate metabolism
- Cellulolytic E. coli : cellulose-> oligo-saccharides-> disaccharide -> glucose(Use ocean microbes which can degrade cellulose to disaccharides, Regulating promoter for find ultimate media.)
- Filamentus bacteria phage
- C. glutamioum: agarose fili designing: new microbes, not E. coli
Next week
- Proteins which can attach to filamentus bacteria phage
Dine together: Le’guette 60500 korean dollars
discussion
discussion
Ideas
- advantage : high effectiveness
- high tolerance enzyme : endothermic
- new substrate-specificity expression
Human practice
Apply Univ-expo
Next week
NGS
Hyukjjin’s presentation
Enzyme fuel cell
reference
Apply Univ-expo
Human practice
Ideas
Next week
Dynamics
Two prey diet choice program.
Two resource diet choice program.
Calorie restriction
Agarose + glucose media : showing calorie restriction
Rock scissor paper theory
Circular GFP
Use various microbes
Parasitic microbes
When the circumstance changes, changing itself will have low effectiveness than living in the nature.
Subject
JI HEE-DNA SEQUENCING
- Maxam-Gilbert sequencing
- Sanger sequencing : chain terminating->Dye-terminator sequencing
- Shotgun sequencing
- Bridge PCR
- Emulsion PCR : DNA fragments are amplified upon primers attached to beads
- 454pyrosequencing
- Illumine sequencing : use bridge PCR
Sponsor
Todo List
- airplane ; H.Y.Choi
- accommodation ; J.H.Park
Todo List
Todo List
Todo List
Todo List
Todo List
Todo List
Todo List
Todo List
Todo List
Todo List
- judging form
- who did experiment, sponsor, advisor
- safety questions
- team collaborations