Team:HokkaidoU Japan/Notebook/H Stem/Replacement To 1C3

From 2014.igem.org

H-Stem Vector Exchange to pSB1A3

  • Start

  • mini-Prep

    1A3 Vector

  • PCR

    Plac-stem-as-stem-dT on 1C3 with 100up-F & 200dn-R

  • Digestion

    1A3 with EcoRI & PstI, Plac-stem-as-stem-dT with EcoRI & PstI

  • Ligation

    1A3 & Plac-stem-as-stem-dT

  • Transformation

    Plac-stem-as-stem-dT on 1A3

  • Complete!

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