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Team:Paris Saclay/Project
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The different parts of our project:
A - The chassis coli Odor free
Escherichia coli stinks because of the tnaA gene which produces an enzyme that transforms the L-tryptophan into indole, responsible for the stench. If we want our lemon to smell like one, we have to delete this gene. In the lab, we already had a strain in which the tnaA was replaced by a kanamycin resistance, but it wasn't the right strain. So we switched the tnaA sequence with the kanamycin resistance in our bacterium by phage transduction. After the recombination, we used a flipase to delete the kanamycin resistance. The remaining bacterium doesn't smell at all.
