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Team:Paris Saclay/Project
From 2014.igem.org
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<li>A - The chassis coli Odor free</li> | <li>A - The chassis coli Odor free</li> | ||
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Escherichia coli stinks because of the tnaA gene which produces an enzyme that transforms the L-tryptophan into indole, responsible for the stench. If we want our lemon to smell like one, we have to delete this gene. | Escherichia coli stinks because of the tnaA gene which produces an enzyme that transforms the L-tryptophan into indole, responsible for the stench. If we want our lemon to smell like one, we have to delete this gene. | ||
In the lab, we already had a strain in which the tnaA was replaced by a kanamycin resistance, but it wasn't the right strain. So we switched the tnaA sequence with the kanamycin resistance in our bacterium by phage transduction. After the recombination, we used a flipase to delete the kanamycin resistance. The remaining bacterium doesn't smell at all. | In the lab, we already had a strain in which the tnaA was replaced by a kanamycin resistance, but it wasn't the right strain. So we switched the tnaA sequence with the kanamycin resistance in our bacterium by phage transduction. After the recombination, we used a flipase to delete the kanamycin resistance. The remaining bacterium doesn't smell at all. | ||
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| - | + | <img src = "https://static.igem.org/mediawiki/2014/f/fc/OdorFree.jpg" alt = "Schema Preparation of the coli odor free chassis" style="width: 400px; height: 500px; /> | |
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<li>B - Construction of the fusion protein (color)</li> | <li>B - Construction of the fusion protein (color)</li> | ||
<li>C - Salicylate Inducible Suppressing System</li> | <li>C - Salicylate Inducible Suppressing System</li> | ||
Revision as of 15:08, 4 August 2014
| Page under construction. Our team is working hard and excited to develop a very good Wiki. | ||
The different parts of our project: |
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