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Team:Paris Saclay/Notebook
From 2014.igem.org
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*DNA extraction from gel agarose - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#C_-_Salicylate_Inducible_Suppressing_System 30th July] | *DNA extraction from gel agarose - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#C_-_Salicylate_Inducible_Suppressing_System 30th July] | ||
*Ligation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#C_-_Salicylate_Inducible_Suppressing_System 30th July] | *Ligation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#C_-_Salicylate_Inducible_Suppressing_System 30th July] | ||
| - | *Transformation | + | *Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#C_-_Salicylate_Inducible_Suppressing_System 31st July] |
| - | + | ||
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1#C_-_Salicylate_Inducible_Suppressing_System 1st August] | *Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1#C_-_Salicylate_Inducible_Suppressing_System 1st August] | ||
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#C_-_Salicylate_Inducible_Suppressing_System 4th August] | *Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#C_-_Salicylate_Inducible_Suppressing_System 4th August] | ||
Revision as of 17:55, 6 August 2014
Contents |
Notebook
We constitute our team in November 2013 and we have meeting twice a month until April. Then we met every weeks. The Lab work started at the beginning of June.
Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work. Click here to edit this page
Planning
C - Salicylate Inducible Suppressing System
- Rehydration of BioBricks BBa_J61051 and BBa_K228001- 23th July
- Transformation of competent E.coli cells - 24th July
- Bacterial culture - 25th July
- Plasmid DNA Purification - 28th July
- Digestion to check & Electrophoresis - 28th July
- Main Digestion - 29th July
- Segregate Process - 29th July
- DNA extraction from gel agarose - 30th July
- Ligation - 30th July
- Transformation of competent E.coli cells - 31st July
- Liquid Culture - 1st August
- Plasmid DNA Purification - 4th August
- Digestion to check & Electrophoresis - 4th August
- Final Stock BBa_K1372000 - 4th August
- Liquid Culture - 5th August
- Plasmid DNA Purification - 6th August
- Digestion of BBa_B0015 and BBa_K1372000 - 6th August
- Segregate Process - 6th August
- DNA extraction from gel agarose - 7th August
- Ligation - 7th August
- Transformation in DH5 alpha - 8th August
- Bacterial Culture - 8th August
- Liquid Culture - 11th August
- Plasmid DNA Purification - 12th August
- Digestion to check & Electrophoresis - 12th August
- Final Stock - 12th August
D - Lemon Scent
- PCR of BBa_517003 and BBa_K762100 - 4th August
- Gel electrophoresis of BBa_K517003 and BBa_K762100 - 5th August
- PCR Cleanup of BBa_K517003 and BBa_K762100 - 6th August
