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Team:Paris Saclay/Notebook
From 2014.igem.org
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==Planning== | ==Planning== | ||
===C - Salicylate Inducible Suppressing System=== | ===C - Salicylate Inducible Suppressing System=== | ||
| - | *Rehydration of BioBricks - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/23#C_-_The_Suppressing_Salicylate_System 23th July] | + | *Rehydration of BioBricks BBa_J61051 and BBa_K228001- [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/23#C_-_The_Suppressing_Salicylate_System 23th July] |
*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#C_-_Salicylate_Inducible_Suppressing_System 24th July] | *Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#C_-_Salicylate_Inducible_Suppressing_System 24th July] | ||
*Bacterial culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25#C_-_Salicylate_Inducible_Suppressing_System 25th July] | *Bacterial culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25#C_-_Salicylate_Inducible_Suppressing_System 25th July] | ||
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#C_-_Salicylate_Inducible_Suppressing_System 28th July] | *Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#C_-_Salicylate_Inducible_Suppressing_System 28th July] | ||
*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#C_-_Salicylate_Inducible_Suppressing_System 28th July] | *Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#C_-_Salicylate_Inducible_Suppressing_System 28th July] | ||
| - | *Main Digestion | + | *Main Digestion - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#C_-_Salicylate_Inducible_Suppressing_System 29th July] |
| - | *DNA | + | *Segregate Process - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#C_-_Salicylate_Inducible_Suppressing_System 29th July] |
| + | *DNA extraction from gel agarose - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#C_-_Salicylate_Inducible_Suppressing_System 30th July] | ||
*Ligation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#C_-_Salicylate_Inducible_Suppressing_System 30th July] | *Ligation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#C_-_Salicylate_Inducible_Suppressing_System 30th July] | ||
*Transformation in DH5 alpha - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#C_-_Salicylate_Inducible_Suppressing_System 31st July] | *Transformation in DH5 alpha - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#C_-_Salicylate_Inducible_Suppressing_System 31st July] | ||
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*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#C_-_Salicylate_Inducible_Suppressing_System 4th August] | *Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#C_-_Salicylate_Inducible_Suppressing_System 4th August] | ||
*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#C_-_Salicylate_Inducible_Suppressing_System 4th August] | *Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#C_-_Salicylate_Inducible_Suppressing_System 4th August] | ||
| - | *Final Stock - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#C_-_Salicylate_Inducible_Suppressing_System 4th August] | + | *Final Stock BBa_K1372000 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#C_-_Salicylate_Inducible_Suppressing_System 4th August] |
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5#C_-_Salicylate_Inducible_Suppressing_System 5th August] | *Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5#C_-_Salicylate_Inducible_Suppressing_System 5th August] | ||
| - | *Plasmid DNA Purification - '' | + | *Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#C_-_Salicylate_Inducible_Suppressing_System 6th August] |
| - | *Digestion - '' | + | *Digestion of BBa_B0015 and BBa_K1372000 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#C_-_Salicylate_Inducible_Suppressing_System 6th August] |
| - | * | + | *Segregate Process - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#C_-_Salicylate_Inducible_Suppressing_System 6th August] |
| + | *DNA extraction from gel agarose - ''7th August'' | ||
| + | *Ligation - ''7th August'' | ||
| + | *Transformation in DH5 alpha - ''8th August'' | ||
| + | *Bacterial Culture - ''8th August'' | ||
| + | *Liquid Culture - ''11th August'' | ||
| + | *Plasmid DNA Purification - ''12th August'' | ||
| + | *Digestion to check & Electrophoresis - ''12th August'' | ||
| + | *Final Stock - ''12th August'' | ||
===D - Lemon Scent=== | ===D - Lemon Scent=== | ||
Revision as of 16:56, 6 August 2014
Contents |
Notebook
We constitute our team in November 2013 and we have meeting twice a month until April. Then we met every weeks. The Lab work started at the beginning of June.
Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work. Click here to edit this page
Planning
C - Salicylate Inducible Suppressing System
- Rehydration of BioBricks BBa_J61051 and BBa_K228001- 23th July
- Transformation of competent E.coli cells - 24th July
- Bacterial culture - 25th July
- Plasmid DNA Purification - 28th July
- Digestion to check & Electrophoresis - 28th July
- Main Digestion - 29th July
- Segregate Process - 29th July
- DNA extraction from gel agarose - 30th July
- Ligation - 30th July
- Transformation in DH5 alpha - 31st July
- Bacterial Culture - 31st July
- Liquid Culture - 1st August
- Plasmid DNA Purification - 4th August
- Digestion to check & Electrophoresis - 4th August
- Final Stock BBa_K1372000 - 4th August
- Liquid Culture - 5th August
- Plasmid DNA Purification - 6th August
- Digestion of BBa_B0015 and BBa_K1372000 - 6th August
- Segregate Process - 6th August
- DNA extraction from gel agarose - 7th August
- Ligation - 7th August
- Transformation in DH5 alpha - 8th August
- Bacterial Culture - 8th August
- Liquid Culture - 11th August
- Plasmid DNA Purification - 12th August
- Digestion to check & Electrophoresis - 12th August
- Final Stock - 12th August
D - Lemon Scent
- PCR of BBa_517003 and BBa_K762100 - 4th August
- Gel electrophoresis of BBa_K517003 and BBa_K762100 - 5th August
- PCR Cleanup of BBa_K517003 and BBa_K762100 - 6th August
