Team:OUC-China/Project

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                    <li><a href="https://2014.igem.org/Team:OUC-China">HOME</a></li>
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                    <li class="dropdown">
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                        <a href="#" class="dropdown-toggle" data-toggle="dropdown">TEAM <b class="caret"></b></a>
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                        <ul class="dropdown-menu">
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                            <li><a href="https://2014.igem.org/Team:OUC-China/Team">Team members</a></li>
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                            <li><a href="https://2014.igem.org/Team:OUC-China/Team_Instructor">Instructor</a></li>
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                            <li><a href="https://2014.igem.org/Team:OUC-China/Team_Acknowledgment">Acknowledgment</a></li>
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                            <li><a href="https://2014.igem.org/Team:OUC-China/Team_Lab">Lab</a></li>
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                            <li><a href="https://igem.org/Team.cgi?year=2014&team_name=OUC-China">Official team profile</a></li>
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                        </ul>
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                    </li>
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                        <a href="#" class="dropdown-toggle" data-toggle="dropdown">PROJECT <b class="caret"></b></a>
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                        <ul class="dropdown-menu">
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                            <li  class="active"><a href="https://2014.igem.org/Team:OUC-China/Project">Overview</a></li>
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                            <li><a href="https://2014.igem.org/Team:OUC-China/Project_Background">Background</a></li>
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                            <li><a href="https://2014.igem.org/Team:OUC-China/Project_Design">Design</a></li>
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                            <li><a href="https://2014.igem.org/Team:OUC-China/Project_Result">Result</a></li>
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                            <li><a href="https://2014.igem.org/Team:OUC-China/Project_Future">Future</a></li>
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                            <li><a href="https://2014.igem.org/Team:OUC-China/Project_Modeling">Modeling</a></li>
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                            <li><a href="https://2014.igem.org/Team:OUC-China/Project_Policy_Practise">Policy&Practice</a></li>
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                        </ul>
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                        <a href="#" class="dropdown-toggle" data-toggle="dropdown">JUDGING <b class="caret"></b></a>
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                            <li><a href="https://2014.igem.org/Team:OUC-China/Judging">Biobrick</a></li>
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                            <li><a href="https://2014.igem.org/Team:OUC-China/Judging_Achievment">Achievement</a></li>
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                        </ul>
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                    </li>
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                        <a href="#" class="dropdown-toggle" data-toggle="dropdown">SAFETY <b class="caret"></b></a>
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                            <li><a href="https://2014.igem.org/Team:OUC-China/Safety">Biosafety</a></li>
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                            <li><a href="https://2014.igem.org/Team:OUC-China/Safety_Lab_safety">Labsafety</a></li>
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                        </ul>
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                    </li>
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                        <ul class="dropdown-menu">
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                            <li><a href="https://2014.igem.org/Team:OUC-China/Notebook">Lab note</a></li>
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                            <li><a href="https://2014.igem.org/Team:OUC-China/Notebook_Modeling_Note">Modeling note</a></li>
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                            <li><a href="https://2014.igem.org/Team:OUC-China/Notebook_Protocols">Protocols</a></li
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<h1 class="text-primary">Overview</h1>
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<img src="https://static.igem.org/mediawiki/2014/d/d7/OUC-China_Project_Overview.png" style="display:block;margin:0 auto" />
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        <p>Generally, researchers transfer plasmids from prokaryote to eukaryote by non-autonomous methods, while we manage to make plasmid transfer by autonomous and convenient methods.</p>
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<p>This year, OUC-China iGEM devotes to design a novel model of plasmid transfer. Because the plasmid can’t transfer into natural bacteria easily in vivo, we transport the plasmid with double plasmids system into the organism, and then the constructed plasmid that has lysis device can transfer to the dominant colony in organism by conjugation. Afterwards, the dominant colony with constructed plasmid can lysis and release the constructed plasmid and a fusion protein composed of cationic TAT peptide and histone H4 that we designed. The protein complex will carry the constructed plasmid into eukaryote to operate. The aim of the project is to construct a novel model method of eukaryotic transfection for molecular biology research at individual level, such as DNA vaccine and molecular marker.</p>
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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:OUC-China/Project&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://2014.igem.org/Team:OUC-China"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:OUC-China/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=OUC-China"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:OUC-China/Project"style="color:#000000"> Project</a></td>
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<a href="https://2014.igem.org/Team:OUC-China/Attributions"style="color:#000000"> Attributions </a></td>
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<p>Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs) </p>
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<h3>References </h3>
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iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you. </p>
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<p> You can use these subtopics to further explain your project</p>
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<li>Overall project summary</li>
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<li>Project Details</li>
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<li>Materials and Methods</li>
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<li>The Experiments</li>
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It's important for teams to describe all the creativity that goes into an iGEM project, along with all the great ideas your team will come up with over the course of your work.  
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It's also important to clearly describe your achievements so that judges will know what you tried to do and where you succeeded. Please write your project page such that what you achieved is easy to distinguish from what you attempted.  
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  <p>contact us: <a href="mailto:oucigem@163.com">oucigem@163.com</a></p>
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Latest revision as of 03:30, 18 October 2014

Overview

Generally, researchers transfer plasmids from prokaryote to eukaryote by non-autonomous methods, while we manage to make plasmid transfer by autonomous and convenient methods.

This year, OUC-China iGEM devotes to design a novel model of plasmid transfer. Because the plasmid can’t transfer into natural bacteria easily in vivo, we transport the plasmid with double plasmids system into the organism, and then the constructed plasmid that has lysis device can transfer to the dominant colony in organism by conjugation. Afterwards, the dominant colony with constructed plasmid can lysis and release the constructed plasmid and a fusion protein composed of cationic TAT peptide and histone H4 that we designed. The protein complex will carry the constructed plasmid into eukaryote to operate. The aim of the project is to construct a novel model method of eukaryotic transfection for molecular biology research at individual level, such as DNA vaccine and molecular marker.

contact us: oucigem@163.com

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