Biobrick

Origin of transfer for the RP4-plasmid nic region

ID: BBa_K1439000

Type: conjugation

Length:350bp

Description/Usage: OriTRP4,the RP4 plasmid nic region,is where the relaxosome nicks the plasmid and conjugative transfer by RP4 plasmid machinery begins.

[OriTRP4]+[RFP]

ID: BBa_K1439001

Type: Composite

Length:1427bp

Description/Usage: This part contains a reporter gene BBa_J04450, combined with OriTRP4. Used to test plasmid mobility.

[OriTR]+[RFP]

ID: BBa_K1439002

Type: Composite

Length:1448bp

Description/Usage: This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.

hEF1a_Cas9-VP16 MammoBlock

ID: BBa_K1179003

Type: Generator

Length: 5141bp

Description/Usage: This part encodes the expression of the fusion protein Cas9-VP16 that is used in activating a minimal CMV promoter with guide RNA binding sites upstream.

TAT-H4

ID: BBa_K1439004

Type: Coding

Length:393bp

Description/Usage: This part codes the expression of the fusion protein TAT-H4 that is used in combing and protecting the plasmid in bacterium and helping the plasmid transfect into the eukaryocyte.

TAT-PTD

ID: BBa_K1439005

Type: Coding

Length:33bp

Description/Usage: This part codes the expression of the TAT-PTD that is used in helping the plasmid transfect into the eukaryocyte.

Histone H4

ID: BBa_K1439006

Type: Coding

Length:312bp

Description/Usage: This part codes the expression of the Histone H4 that is used in combing and protecting the plasmid in bacterium.

CMV promoter

ID: BBa_K1439007

Type: Regulatory

Length:588bp

Description/Usage: We use CMV to improve the expression of TAT:H4 and the efficiency of transfection.

TAT-H4-B0015

ID: BBa_K1439008

Type: Composite

Length:530bp

Description/Usage: This part codes the expression of the fusion protein TAT-H4 that is used in combing and protecting the plasmid in bacterium and helping the plasmid transfect into the eukaryocyte. It has a terminator and can be added different promoters.

Lysis device induced by L-arabinose

ID: BBa_K1439009

Type: Device

Length:1923bp

Description/Usage:This part encodes the expression of the lysis protein that is induced by L-arabinose mobility.

Lysis device induced by aTc

ID: BBa_K1439010

Type: Device

Length:2738bp

Description/Usage:This is the lysis device using three genes from enterobacteria phage T4: lysozyme, holin, and antiholin.Tet promoter can control the lysis of bacteria.