Team:London BioHackspace/Printer Hardware

From 2014.igem.org

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The basic proposal for juicyprint is the growth of cellulose by bacteria under the control of light. The 660nm light that the Cph1 protein is sensitive to inhibits the growth. Therefore we designed a system to generate the production of a pellicle sheet in the shape of a projected template of the negative image.
The basic proposal for juicyprint is the growth of cellulose by bacteria under the control of light. The 660nm light that the Cph1 protein is sensitive to inhibits the growth. Therefore we designed a system to generate the production of a pellicle sheet in the shape of a projected template of the negative image.
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|So a light pattern like this
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|should result in a pellicle growth pattern like this
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|[[File:Hackspace logo.png|200px]]
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|[[File:Hackspace logo2dwqdwqdwq.png|200px]]
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==Aim==
==Aim==

Revision as of 00:33, 18 October 2014

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Contents

Intro

15259724148 b72dec6fe7 b.jpg

The basic proposal for juicyprint is the growth of cellulose by bacteria under the control of light. The 660nm light that the Cph1 protein is sensitive to inhibits the growth. Therefore we designed a system to generate the production of a pellicle sheet in the shape of a projected template of the negative image.

So a light pattern like this should result in a pellicle growth pattern like this
Hackspace logo.png Hackspace logo2dwqdwqdwq.png

Aim

The aim is to provide a basic proof of concept to test how the light sensitive bacteria respond to the stimulus.


Methods

We were unable to fully test the system with the target system. Therefore we simulated the process with just a liquid target.

660nm light is a bright red colour;

Image002igh875yt785y87t5y4.jpg

Image courtesy of Beer Color Laboratories; http://www.beercolor.com/color_basics1.htm


Results

A thick 660nm camera filter gets very hot when placed near the projector for a period of time.

Therefore I settled on using a home-made red permanent marker pen to make a 35mm slide suitable for projection. The slide was much thinner and didn't absorb so much light and get hot.