Team:Heidelberg/Toolbox/Induction

ABSTRACT

Protein splicing allows for the introduction of major changes to a proteins sequence and structure even after translation. Although this mechanism alone offers a huge amount of possible applications, it is of advantage to have control over the time and location of the splicing reaction especially for in vivo applications. To gain this control there are several approaches: For in vitro reactions this is quite easy: Just mix your samples to protein splicing. If it needs to be more elaborate, many inteins exist that can be controlled by the pH-value or the redox-state of their environment. we have tried to develop a set of light-inducible intein-systems using the Avena Sativa LOV2 domain.