Team:Heidelberg

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<a href="https://2014.igem.org" style="position:absolute;z-index:1;"><img src="/wiki/images/5/5d/IGEM_logo_white.png" style="height:70px;" alt="iGEM Logo" /></a>
<a href="https://2014.igem.org" style="position:absolute;z-index:1;"><img src="/wiki/images/5/5d/IGEM_logo_white.png" style="height:70px;" alt="iGEM Logo" /></a>
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<div class="col-lg-6 col-md-6 col-sm-12 impressiv">
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<div class="hidden-sm hidden-xs" style="padding-top:100px;"></div>
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<h3>iGEM TEAM <span>HEIDELBERG</span> 2014</h3>
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<h3 class="normal-medium-text">iGEM TEAM <span>HEIDELBERG</span> 2014</h3>
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<h1>THE RING<br />OF <span>FIRE</span></h1>
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<h1 class="very-large-text">THE RING<br />OF <span>FIRE</span></h1>
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<p>Welcome to the proteins of tomorrow.</p>
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<p>This is the iGEM team Heidelberg‘s wiki page.</p>
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<p style="font-size:30px"> Click <a href="/Team:Heidelberg/Project#Abstract">here</a> to view our abstract.
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<br/><p> Click here to view out abstract.</p>
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<br/>
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<p>Scroll down to explore our project.
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Scroll down to EXPLORE our project.
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<h1>Hallo, das ist ein test</h1>
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<p><span class="larger red-text">Nature</span> has made many curious inventions. One of these are</p>
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<p>dhsfjkask sdhfasdf sdhfhsagfdjh sdafjhsgdjfh sdgfhasgjf sdfhagjsd jf jsdgfhgj jdf jgj jhgdhfgsh hghjsdgfjkajgjhfd jajhdgfjsa</p>
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<h1 class="very-large-text red-text bold" style="text-align:right;"><a href="https://2014.igem.org/Team:Heidelberg/Toolbox/Circularization">CIRCULAR PROTEINS</a></h1>
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<p>dhsfjkask sdhfasdf sdhfhsagfdjh sdafjhsgdjfh sdgfhasgjf sdfhagjsd jf jsdgfhgj jdf jgj jhgdhfgsh hghjsdgfjkajgjhfd jajhdgfjsa</p>
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<p>which are unconventional peptides that neither have a beginning, nor an ending</p><br>
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<p>dhsfjkask sdhfasdf sdhfhsagfdjh sdafjhsgdjfh sdgfhasgjf sdfhagjsd jf jsdgfhgj jdf jgj jhgdhfgsh hghjsdgfjkajgjhfd jajhdgfjsa</p>
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<p class="normal-medium-text bold">These proteins are extremely <span class="red-text">resistant</span> against
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<p><a class="btn btn-primary btn-lg" role="button">Learn more</a></p>
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<span class="red-text">high temperatures</span>, pH changes and proteases.</p>
 +
<p class="normal-medium-text align-right">We established protein <a href="/Team:Heidelberg/Toolbox/Circularization"> circularization </a> as a new<br>powerful tool for Synthetic Biology and set the foundations<br>to render any protein heat stable.</p>
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<h1>Hallo, das ist ein test</h1>
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<h1 class="large-text bold">Wondering how we circularize?</h1>
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<p>dhsfjkask sdhfasdf sdhfhsagfdjh sdafjhsgdjfh sdgfhasgjf sdfhagjsd jf jsdgfhgj jdf jgj jhgdhfgsh hghjsdgfjkajgjhfd jajhdgfjsa</p>
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<h2 class="normal-medium-text align-right bold">Let us introduce you to the next generation of bioengineering...</h2>
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<p><a class="btn btn-primary btn-lg" role="button">Learn more</a></p>
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<h2 class="align-right" style="font-size: 40px; font-weight:normal">come DISCOVER the <a href="/Team:Heidelberg/Project/Background">MECHANISM of SPLIT INTEINS</a></h2><br><br>
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<p class=""><span class="larger">Inteins excise themselves out of proteins and in doing so the remaining flanking parts are irreversibly joined</span></p>
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<div class="col-lg-4 col-lg-push-8" style="z-index:5;">
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<p><span class="larger">–&nbsp;an effective mechanism to circularize proteins.</span></p>
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<img class="img-responsive" src="/wiki/images/a/a6/Heidelberg_Project_Dnmt1.png" />
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<h1 class="dark-grey-text" style="text-align: right;"><span style="font-size: 0.8em;">circular <span class="red-text">heat-stable</span></span><br>DNMT1</h1>
+
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<p>Wouldn´t it be great to amplify DNA in a normal PCR maintaining the epigenetic information coded in methylation patterns?</p>
+
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<p>The problem: DNMT I, an enzyme which is responsible for the establishment and maintenance of the individual methylation pattern of different cell types, is not heat stable.
+
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For iGEM 2014 we therefore create a PCR 2.0 with heat-stable DNMT I by circularization.
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</p>
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<span class="title-dnmt1">PCR 2.0</span>
 
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<span class="special-span-dnmt1"></span>
 
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<img class="img-responsive" src="/wiki/images/8/82/Heidelberg_Project_Xylanase.png" />
 
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<h1 style="text-align: right; color: black; padding-right: 130px;" ><span style="font-size: 0.8em;">circular <span style="color:white;">heat-stable</span></span><br><span style="font-size:1.3em;">Xylanase</span></h1>
 
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<p>Xylanase is an important enzyme for the pulp and paper industry.</p>
 
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<p>Bla bla</p>
 
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<p>In future Xylanase could be used for the production of biofuel.</p>
 
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<div class="title-wrapper-xylanase" style="text-align: right; color:white;">
 
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<span class="title-xylanase">INDUSTRY</span>
 
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<span class="special-span-xylanase"></span>
 
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<div class="jumbotron slide darker-background modeling-image-bg" style="color:white;">
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<div>    
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<div class="col-xs-12">
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<img class="img-responsive" src="/wiki/images/d/df/Heidelberg_Lysozyme.png" />
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<p class="normal-medium-text">But messing with protein structure can be disastrous,<br><span class="medium-text">you can only win with good <a class="bold" href="/Team:Heidelberg/Modeling">MODELING</a>.</span></p><br><br>
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</div>
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<p class="normal-medium-text"><span>Find out the <span class="bold">EXCITING THEORY</span><br>behind <span class="bold">RIGID LINKERS</span> and their <span class="bold">ANGLES</span></span></p>
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<div class="col-lg-8 col-lg-pull-4">
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<br>
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<h1 style="text-align: left;" ><span style="font-size:1.3em;">LINK it!</span></h1>
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<p><span class="larger">We developed <a href="/Ream:Heidelberg/Software/Linker_Software">CRAUT</a>,<br>
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<p>Could every protein becomes heat stable by circularization even if it´s the complexest of all?</p>
+
a comprehensive software which identifies<br>
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<p>Circularization is a narrow path between heat-stability and loss of function due to deformation. We developed a linker software, which predict the most suitable linker depending on the folding structure of every protein.</p>
+
the optimal path to connect a protein’s termini<br>
 +
preserving structure and function.</span></p>
</div>
</div>
<div class="col-lg-12">
<div class="col-lg-12">
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<p>In an extensive linker screening our software was improved and calibrated using the lambda phage lysozyme.</p>
+
<p><span class="larger">Look at our extensive wet-lab SCREENING to improve and calibrate our software using <a class="red-text" href="/Team:Heidelberg/Project/Linker_Screening">lambda lysozyme</a>!</span></p>
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<img class="img-responsive" src="/wiki/images/e/e5/Heidelberg_Frontpage_igemathome.png" />
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<a href="/Team:Heidelberg/Software/igemathome"><img class="img-responsive" src="/wiki/images/e/e5/Heidelberg_Frontpage_igemathome.png"></a>
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<div class="col-md-8 col-xs-12">
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<h1 style="text-align: left; color:#DE4230;" ><span style="font-size:1.3em;">CALCULATE it!</span></h1>
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<p class="align-right"><span class="larger">As calculations on protein structures are costly<br>we involved the rest of the world with</span></p>
 +
<h1 class="align-right bold very-large-text dark-red-text"><span style="font-size:1.5em;"><a href="/Team:Heidelberg/Human_Practice/igemathome">iGEM@home</a></span></h1>
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<div class="clearfix"></div>
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<div class="col-md-8 col-xs-12">
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<p>After calculating eleven days and the breakdown of both computational and mental power we decided to spread the modeling of the linkers.</p>
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<h3 class="medium-text bold">Empowering science!</h3><br>
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<p>The iGEM Team Heidelberg developed <a href="/Team:Heidelberg/Software/igemathome">iGEM@home</a>, a software to divide extensive computing task into many packages and to distribute them to many computers. Now over 1.000 volunteers are calculating for us when their are idle.</p>
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<p class="normal-medium-text"><a href="/Team:Heidelberg/Software/igemathome">NEW PLATFORM</a> for distributed computing.</p>
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<p>As a new tool for the iGEM community this system enables every student team to archieve their modeling without access to big server farms.</p>
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<p><span class="larger">Volunteers provide the idle calacity of their home computers.</span></p>
 +
<p class=""><span class="larger">And, with an established user base with more than <span class="larger red-text">1000</span> computers, we effectively <a href="/Team:Heidelberg/Human_Practice/igemathome" >bring synthetic biology to society</a> in a new way!</span></p>
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<img style="position:relative; top: -35px;" class="img-responsive" src="/wiki/images/5/52/Heidelberg_Frontpage_igemathome_cloud.png" />
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<a href="/Team:Heidelberg/Human_Practice/igemathome" ><img style="position:relative; top: -35px;" class="img-responsive" src="/wiki/images/5/52/Heidelberg_Frontpage_igemathome_cloud.png"></a>
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<div class="arrow arrow-right"></div>
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<div>
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<div class="col-md-4 col-md-push-8 col-md-offset-0 col-xs-offset-3 col-xs-6" style="z-index:5;">
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<a href="/Team:Heidelberg/Project/PCR_2.0" ><img class="img-responsive" src="/wiki/images/a/a6/Heidelberg_Project_Dnmt1.png"></a>
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</div>
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<div class="col-md-8 col-md-pull-4 col-xs-12">
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<h1 class="large-text">Our Application:</h1>
 +
<h1 class="dark-grey-text large-text" style="text-align: right;"><span class="medium-large-text">circular <a href="/Team:Heidelberg/Project/PCR_2.0">heat-stable</a></span><br>DNA-methyltransferase</h1>
 +
<br><br><p><span class="larger">Wouldn´t it be great to amplify DNA preserving the encoded epigenetic information?</span></p>
 +
<p><span class="larger">This could lead to an ENTIRELY NEW way of performing PCRs with a <a href="/Team:Heidelberg/Project/PCR_2.0">heat stable methyltransferase</a>!</span></p>
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<p>&nbsp;<br /><span class="larger">We'd like to call it</span></p>
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</div>
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<div class="clearfix"></div>
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<div>
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<div class="title-wrapper-dnmt1">
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<a href="/Team:Heidelberg/Project/PCR_2.0" class="title-dnmt1">PCR 2.0</a>
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<span class="special-span-dnmt1"></span>
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<div class="arrow arrow-left"></div>
<div class="arrow arrow-left"></div>
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<div class="col-lg-4 col-md-4 col-xs-12" style="height: 410px; position: relative">
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<div id="circ-box" class="descr-box">
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<a href="https://2014.igem.org/Team:Heidelberg/Project/Toolbox#Circularization">
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<img src="/wiki/images/5/58/Heidelberg_Toolbox_Circularization.png" id="circ-icon" class="toolbox-icon">&nbsp;&nbsp;CIRCULARIZATION
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</a>
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</div>
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<div id="oligo-box" class="descr-box">
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<a href="https://2014.igem.org/Team:Heidelberg/Project/Toolbox#Oligomerization">
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<img src="/wiki/images/4/40/Oligomerization.png" id="oligo-icon" class="toolbox-icon">&nbsp;&nbsp;OLIGOMERIZATION
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</a>
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</div>
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<div id="fusion-box" class="descr-box">
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<a href="https://2014.igem.org/Team:Heidelberg/Project/Toolbox#Fusion_and_Tagging">
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<img src="/wiki/images/8/87/Heidelberg_Toolbox_Fusion.png" id="fusion-icon" class="toolbox-icon">&nbsp;&nbsp;FUSION
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</a>
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</div>
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<div id="onoff-box" class="descr-box">
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<a href="https://2014.igem.org/Team:Heidelberg/Project/Toolbox#On_Off">
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<img src="/wiki/images/c/c2/Heidelberg_Toolbox_On-Off.png" id="onoff-icon" class="toolbox-icon">&nbsp;&nbsp;ON/OFF
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</a>
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</div>
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<div id="purification-box" class="descr-box">
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<a href="https://2014.igem.org/Team:Heidelberg/Project/Toolbox#Purification">
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<img src="/wiki/images/0/04/Heidelberg_Toolbox_Purification.png" id="purification-icon" class="toolbox-icon">&nbsp;&nbsp;PURIFICATION
 +
</a>
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</div>
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<div class="col-lg-8 col-md-8 col-xs-12">
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<h2 class="normal-medium-text align-right">One more thing.<br>Inteins are capable of much more!</h2>
 +
<h1 class="medium-large-text align-right bold">We show you the world of</h1>
 +
<h1 class="medium-text align-right red-text bold" style="font-size: 41px;">post-translational MODIFICATIONS</h1><br><br>
 +
 +
<p class="align-right "><span class="larger">We created an <a href="/Team:Heidelberg/Project/Toolbox">INTEIN TOOLBOX</a> so you can easily modify your protein in a standardized way.</span></p>
 +
<p class="align-right"><span class="larger">Take a look at our <a href="/Team:Heidelberg/Parts/RFC">RFC</a>!</span></p>
 +
<p class="align-right"><span class="larger">Explore all our tools!</span></p>
 +
</div>
 +
<div class="clearfix"></div>
 +
<div class="arrow arrow-right"></div>
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</div>
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</div>
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<div class="row">
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<div class="col-lg-5 col-md-5 hidden-sm hidden-xs" style="position:relative; bottom:-48px;">
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<img class="img-responsive" src="/wiki/images/5/5e/Heidelberg_Frontpage_Team.png" />
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</div>
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<div class="col-lg-7 col-md-7 col-sm-12 col-xs-12">
 +
<h1>Who are we?</h1>
 +
<p>We are the iGEM Team Heidelberg 2014 consisting of 12 highly motivated bachelor and master students studying at Heidelberg University.</p>
 +
<p>For our project we got great feedback and support from our supervisors.</p>
 +
<p>Take a look at our <a href="/Team:Heidelberg/Team">Teampage</a>!</p>
 +
<h1>Thank you!</h1>
 +
<p>We want thank all people who helped us and supported our work in the lab.</p>
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</div>
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Latest revision as of 23:43, 16 October 2014

iGEM Logo
Ring of fire Image

iGEM TEAM HEIDELBERG 2014

THE RING
OF FIRE

Click here to view our abstract.
Scroll down to EXPLORE our project.

Nature has made many curious inventions. One of these are

CIRCULAR PROTEINS

which are unconventional peptides that neither have a beginning, nor an ending


These proteins are extremely resistant against high temperatures, pH changes and proteases.

We established protein circularization as a new
powerful tool for Synthetic Biology and set the foundations
to render any protein heat stable.

Wondering how we circularize?

Let us introduce you to the next generation of bioengineering...

come DISCOVER the MECHANISM of SPLIT INTEINS



Inteins excise themselves out of proteins and in doing so the remaining flanking parts are irreversibly joined

– an effective mechanism to circularize proteins.

But messing with protein structure can be disastrous,
you can only win with good MODELING.



Find out the EXCITING THEORY
behind RIGID LINKERS and their ANGLES


We developed CRAUT,
a comprehensive software which identifies
the optimal path to connect a protein’s termini
preserving structure and function.

Look at our extensive wet-lab SCREENING to improve and calibrate our software using lambda lysozyme!

As calculations on protein structures are costly
we involved the rest of the world with

iGEM@home

Empowering science!


NEW PLATFORM for distributed computing.

Volunteers provide the idle calacity of their home computers.

And, with an established user base with more than 1000 computers, we effectively bring synthetic biology to society in a new way!

Our Application:

circular heat-stable
DNA-methyltransferase



Wouldn´t it be great to amplify DNA preserving the encoded epigenetic information?

This could lead to an ENTIRELY NEW way of performing PCRs with a heat stable methyltransferase!

 
We'd like to call it

One more thing.
Inteins are capable of much more!

We show you the world of

post-translational MODIFICATIONS



We created an INTEIN TOOLBOX so you can easily modify your protein in a standardized way.

Take a look at our RFC!

Explore all our tools!

Who are we?

We are the iGEM Team Heidelberg 2014 consisting of 12 highly motivated bachelor and master students studying at Heidelberg University.

For our project we got great feedback and support from our supervisors.

Take a look at our Teampage!

Thank you!

We want thank all people who helped us and supported our work in the lab.

Retrieved from "http://2014.igem.org/Team:Heidelberg"