Team:Evry/Model/pcb model

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IGEM Evry 2014

Pcb Model

PCB sensor model


Introduction

After considering the water flux and pollutant motions in our sponge, we investigated the sensing power of our PCB construction.
We first modelled the behaviour of GFP production regarding PCB absorption to determine if our sensing strategy was viable.

Model description

Goal

We know that BphR1 promoter is induced by the complex BphR2-PCB. We needed to know that at
The PCB pollution have been identify as a major problem in the ocean.
We wanted to tackle this issue by using a sensing device integrated into a synbiotic bacteria of sponges [link to description].
This approach raise few questions:
  • How does our device behave in presence of PCB?
  • What are the lowest or highest PCB concentration detectable?
  • Considering the symbiosis with the sponge, how could our device be disturbed?