Team:Evry/Model/pcb model

From 2014.igem.org

(Difference between revisions)
 
(13 intermediate revisions not shown)
Line 12: Line 12:
After considering the water flux and pollutant motions in our sponge, we investigated the sensing power of
After considering the water flux and pollutant motions in our sponge, we investigated the sensing power of
<a href="https://2014.igem.org/Team:Evry/Biology/Sensors#PCB_construct">our PCB construction</a>. <br/>
<a href="https://2014.igem.org/Team:Evry/Biology/Sensors#PCB_construct">our PCB construction</a>. <br/>
-
We first modelled the behaviour of GFP production regarding PCB absorption to determine if our sensing strategy was viable.
+
We first modelled the behaviour of RFP production regarding PCB absorption to determine if our sensing strategy was viable.
 +
However, we previously tried to create a particle system of our system in the cell.
<h3> Model description </h3>
<h3> Model description </h3>
-
<!--A description of the production of fluorescent protein (GFP)-->
+
<!--A description of the production of fluorescent protein (RFP)-->
-
According to our device, GFP production is controlled by BphR1 promoter. This promoter is induced by the BphR2-PCB complex.  
+
<!---According to our device, RFP production is controlled by BphR1 promoter.
-
Thus our GFP production will depend on the dynamic of BphR2-PCB complex. <br/>
+
This promoter is induced by the BphR2-PCB complex.
-
We used a simple description of the GFP production.  
+
Thus our RFP production will depend on the dynamic of BphR2-PCB complex.  
-
We know that BphR1 promoter is induced by the complex BphR2-PCB. We needed to know that at <br/>
+
On one hand, we used a simple description of the RFP production.<!--, on the other hand we described the dynamic of BphR2-PCB complex with respect to BphR2, PCB, and plasmid concentration.--> <br/>
 +
<!--<img src="https://static.igem.org/mediawiki/2014/f/fe/Team_EVRY_GFP_eqn.png"/>-->
-
<!-- At a cellular level, we designed a simple model to observe the GFP production of -->
+
<img src="https://static.igem.org/mediawiki/2014/7/7b/Team_Evry_figure_1.png"/>
-
The PCB pollution have been identify as a major problem in the ocean.</br>
+
<!-- The PCB pollution have been identify as a major problem in the ocean.</br>
We wanted to tackle this issue by using a sensing device integrated into a synbiotic bacteria of sponges [link to description]. </br>
We wanted to tackle this issue by using a sensing device integrated into a synbiotic bacteria of sponges [link to description]. </br>
This approach raise few questions:
This approach raise few questions:
Line 30: Line 32:
  <li> What are the lowest or highest PCB concentration detectable?
  <li> What are the lowest or highest PCB concentration detectable?
  <li> Considering the symbiosis with the sponge, how could our device be disturbed?
  <li> Considering the symbiosis with the sponge, how could our device be disturbed?
-
</ul>
+
</ul>-->
</div>
</div>
<br>
<br>
-
 
  </div>
  </div>
</div>
</div>

Latest revision as of 04:00, 18 October 2014

IGEM Evry 2014

Pcb Model

PCB sensor model


Introduction

After considering the water flux and pollutant motions in our sponge, we investigated the sensing power of our PCB construction.
We first modelled the behaviour of RFP production regarding PCB absorption to determine if our sensing strategy was viable. However, we previously tried to create a particle system of our system in the cell.

Model description