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Team:Evry/Model/pcb model
From 2014.igem.org
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Revision as of 02:05, 18 October 2014
Pcb Model
PCB sensor model
Introduction
After considering the water flux and pollutant motions in our sponge, we investigated the sensing power of
our PCB construction.
We first modelled the behaviour of GFP production regarding PCB absorption to determine if our sensing strategy was viable.
Model description
According to our device, GFP production is controlled by BphR1 promoter.
This promoter is induced by the BphR2-PCB complex.
Thus our GFP production will depend on the dynamic of BphR2-PCB complex.
We used a simple description of the GFP production. We know that BphR1 promoter is induced by the complex BphR2-PCB. We needed to know that at
The PCB pollution have been identify as a major problem in the ocean. We wanted to tackle this issue by using a sensing device integrated into a synbiotic bacteria of sponges [link to description]. This approach raise few questions:
This promoter is induced by the BphR2-PCB complex.
Thus our GFP production will depend on the dynamic of BphR2-PCB complex.
We used a simple description of the GFP production. We know that BphR1 promoter is induced by the complex BphR2-PCB. We needed to know that at
The PCB pollution have been identify as a major problem in the ocean. We wanted to tackle this issue by using a sensing device integrated into a synbiotic bacteria of sponges [link to description]. This approach raise few questions:
- How does our device behave in presence of PCB?
- What are the lowest or highest PCB concentration detectable?
- Considering the symbiosis with the sponge, how could our device be disturbed?
