Team:Evry/Interlab Study/Used Devices

From 2014.igem.org

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<br> Constructions were built using classical Biobrick restriction sites.  
<br> Constructions were built using classical Biobrick restriction sites.  
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Revision as of 20:48, 17 October 2014

IGEM Evry 2014

Interlab study - Used devices

Used Biobricks and plasmids



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Plasmid map 1: PSB1C3 map with restriction sites (NotI, BsaI, EcoRI, PstI, SpeI and XbaI)

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Plasmid map 2: BBa_J61002 plasmid map with restriction sites and J23101 promoter ( EcoRI, PstI, SpeI and XbaI)

Anderson collection promoter sequences and mutations.
Promoter name, plate and well location into the iGEM distribution kit 2014, vector, resistance cassette, part size in bp, used primers and PCR product profile.

Construction way


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Constructions were built using classical Biobrick restriction sites.

  • Required device 1: originally this device was carried by PSB1K3 and was sud-cloned onto PSB1C3.
    • Vector: digestion of PSB1C3 and PSB1K3 carrying I20260 via EcoRi and PstI
    • Insert: digestion of PSB1K3 carrying I20260 via EcoRi and PstI
    • Ligation with T4 DNA ligase (New England Biolabs)
  • Required device 2, 3 and the 19 constructions for the entire Anderson library: promoters were inserted into PSB1C3 carrying E0240. Process is described on following figure 1.
    • Vector: digestion of PSB1C3 carrying E0240 via EcoRI and XbaI
    • Insert: digestion of PSB1C3 carrying K823012* and J61002 carrying J23100 to J23119 via EcoRI and SpeI
    • Ligation with T4 DNA ligase (New England Biolabs)
* BBa_K823012 corresponds to BBa_J23115 that contains 2 missmatched basepairs.

Analysis protocol


Our team decided to test the fluorescence with a TECAN infiniteM200 in 96 well plates. Each assay were performed after a 16 - 18 h preculture on 5 ml of LB medium at 37°C with 220 rpm agitation. Cultures were distributed 200 µl per well. Three strains per constructions were tested in five replicates. Two controls were added on plates: LB with chloramphenicol and the strain carrying the promoterless vector on LB with chloramphenicol.
Fluorescence data were analyzed via following formula that come from 2010 De Jong et al Experimental and computational validation of models of fluorescent and luminescent reporter genes in bacteria .

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Equation A: A(t) is the corrected absorbance of DH5 alpha with the functional reporter system, Au(t)the uncorrected absorbance of DH5 alpha with the functional reporter system and Ab(t)the background absorbance corresponding to LB with chloramphenicol medium absorbance.
Equation B: B(t) is the absorbance of the strain carrying the promoterless vector, Iu(t) the uncorrected fluorescence intensity of DH5 alpha with the functional reporter system and Ib(t) the background fluorescence intensity of LB chloramphenicol medium with the DH5 alpha carrying the promoterless vector.