The goal of the interlab study is to obtain GFP fluorescence data from the iGEM teams all around the world for about twenty constructions. This will permit to observe the result repeatability across technics, strains and teams.

Anderson collection promoter sequences and mutations.

There were two sub-parts in this study: The mandatory one: obtain fluorescence data for three specific genetic devices expressing GFP and compare them. (donner les constructions) (J23100 to J23119). The Extra Credit assignments: we choose to study the entire Anderson library of constitutive prokaryotic promoters. This library corresponds to 19 constitutive promoters containing some nucleotide mutations on the -35 and the -10, as shown figure x. The idea is to compare the expression strength of promoters according to mutations, from the maximum amount of fluorescence data in order to have significant results. To test the expression of promoters, they are cloned into the PSB1C3 vector followed by the BBa_E0240 , a GFP generator. Constructions are inserted in E. coli DH5 alpha that grown on LB medium added with the selection agent, chloramphenicol. Our team decided to test the fluorescence with a TECAN infiniteM200 in 96 well plates.