Team:Colombia/Interlab

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Interlab Study

This year Colombia Team-iGEM is participating in Interlab Study! We are glad of share our results and measures. You can find them in the tabs below, you can find the specifications of our equipments and the methodology we used as well.

Methods

Text overview

Contents

Methods

Method1

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Method2

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Validation

The following gels show respectively the plasmids with the devices and the restriction profiles for each of the tree devices digested with EcoRI and PstI. For restrictions we obtained two bands (the plasmid and the device) with the expected sizes in all cases.

GELES :)

You can check the size of each device with the next table:

Primers used to construct XXXXXXX
Name Sequence
FW pqrr4 CTT GTC TAG ATT GCA TTT TGC AAA TTT TG
RV pqrr4 GGT CAC TAG TCA ATC TAA TAA ATG CAG ATT
FW LuxOD47E CAA TTC TAG AAA AGA GGA GAA ATA CTA GAT G
RV LuxOD47E ATA TAC TAG TTG TGA CTC TAG TAG AGA

Measures

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Fluorescence microscopy

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Fluorometry

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Measures

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Fluorescence microscopy

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Fluorometry

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Table2. Stress related cortisol levels form male, female and mean. (Kupfer, 1996)

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Figure 1. Scatter Plot of changes in Cortisol Levels (nmol/L) in Males, depending on the hour of the day.

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Figure 2. Scatter Plot of changes in Cortisol Levels (nmol/L) in Females, depending on the hour of the day.

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Figure 3. Scatter Plot of changes in Cortisol Levels (nmol/L) in the Mean between Males and Females, depending on the hour of the day with shaded area of threshold identified.


Measures

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Fluorescence microscopy

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Fluorometry

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