Team:BostonU/Workflow

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Workflow

Phase I - Build and test basic parts.

Key software tools: TASBE Tools, Eugene (optional), Raven (optional)

General Chimera Workflow

Case Study: BU Priority Encoder

general I

• Add parts to MoClo library. These parts were found to be necessary for our priority encoder:

• 3 MoClo level 1 and 3 MoClo level 2 backbones, each with a different origin of replication:

• ColE1
• p15A
• pSC101

• X MoClo level 0fusion proteins:

• TetR_GFP
• ...

• X MoClo level 0tandem promoters:

• pTet_pLac
• ...

These parts were cloned into a E. coli Bioline strain using our MoClo and transformation protocols. They were purified, sequenced, and tested using our FACS Workflow. These parts were then cloned into appropriate vectors and tested in our Fortessa flow cytometer. The TASBE Tools were then employed to characterize their expression.

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