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Team:BostonU/Workflow
From 2014.igem.org
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<th scope="col" colspan="2"><h2>Phase I - Build and test basic parts.</h2> | <th scope="col" colspan="2"><h2>Phase I - Build and test basic parts.</h2> | ||
| + | </th></tr> | ||
| + | <tr> | ||
| + | <th scope="col" colspan="2"><h3>Key software tools: TASBE Tools, Eugene (optional), Raven (optional)</h3> | ||
</th></tr> | </th></tr> | ||
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| - | <p class="tab">• X <a href="https://2014.igem.org/Team:BostonU/FusionProteins">fusion proteins</a>:<br></p> | + | <p class="tab">• X MoClo level 0<a href="https://2014.igem.org/Team:BostonU/FusionProteins">fusion proteins</a>:<br></p> |
<p class="dtab"> | <p class="dtab"> | ||
• TetR_GFP<br> | • TetR_GFP<br> | ||
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</p> | </p> | ||
| - | <p class="tab">• X <a href="https://2014.igem.org/Team:BostonU/ProjectTandemPromoters">tandem promoters</a>:<br> | + | <p class="tab">• X MoClo level 0<a href="https://2014.igem.org/Team:BostonU/ProjectTandemPromoters">tandem promoters</a>:<br> |
<p class="dtab"> | <p class="dtab"> | ||
• pTet_pLac<br> | • pTet_pLac<br> | ||
• ...<br><br> | • ...<br><br> | ||
</p> | </p> | ||
| - | + | <p class="tab">These parts were cloned into a <italic>E. coli</italic> Bioline strain using our MoClo and transformation protocols. They were purified, sequenced, and tested using our FACS Workflow. These parts were then cloned into appropriate vectors and tested in our Fortessa flow cytometer. The TASBE Tools were then employed to characterize their expression.</p> | |
</th> | </th> | ||
Revision as of 17:45, 23 September 2014
Phase I - Build and test basic parts. | |
|---|---|
Key software tools: TASBE Tools, Eugene (optional), Raven (optional) | |
General Chimera Workflow |
Case Study: BU Priority Encoder |
| general I |
• Add parts to MoClo library. These parts were found to be necessary for our priority encoder: • 3 MoClo level 1 and 3 MoClo level 2 backbones, each with a different origin of replication:
• ColE1 • X MoClo level 0fusion proteins:
• TetR_GFP • X MoClo level 0tandem promoters:
• pTet_pLac These parts were cloned into a |
