Team:BostonU/Measurement

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Revision as of 02:09, 16 October 2014

Measurement Track

Why is measurement important?

Synthetic Biology is a relatively new field of research. It involves the assembly of nucleic acid polymers in many possible arrangements and checking for the range of function that they exhibit. Thus, efficient measurement of genetic devices becomes integral to determining their function.

Why is BU in the measurement track?

The BU Chimera workflow [link] efficiently combines software with wet lab protocols to yield complex devices. In using this workflow, one goes around the standard design-build-test cycles at least thrice, which are referred to as different phases [??]. Testing for any device involves the use of TASBE Tools and flow cytometry. This is used extensively in Phase 2 of the workflow as one multiplexing reaction can potentially yield many possible constructs. Here, measurement can be used to differentiate between a plethora of constructs and to pick the best combinatorial possibility for the assembly of the larger device. Measurement, hence, is an essential part of the Chimera workflow as the results from one phase are necessary as the inputs in the next phase.

Considerations in measurement strategy

Strength of E. Coli

Bioline Strain vs Pro Strain

Testing Strain?

Standardized Data for sharing and reproduction

Another one of our goals for iGEM was to obtain and present standard characterized data that can then be compared with data in other labs. This goes back to the reason why we wanted to incorporate software tools into the design-build-test cycle. We wanted to achieve predictive design using cost-effective methods. This will only be possible if all labs in the Synthetic Biology community are able to compare each other’s data and that is why we use the TASBE tools, which are available for the entire community and convert arbitrary fluorescent data to standard Molecules of Equivalent Fluoresein (MEFLs)

Our Sponsors

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 <h3>Testing Strain?</h3>
 <h3> Standardized Data for sharing and reproduction </h3>
+Another one of our goals for iGEM was to obtain and present standard characterized data that can then be compared with data in other labs. This goes back to the reason why we wanted to incorporate software tools into the design-build-test cycle. We wanted to achieve predictive design using cost-effective methods. This will only be possible if all labs in the Synthetic Biology community are able to compare each other’s data and that is why we use the TASBE tools, which are available for the entire community and convert arbitrary fluorescent data to standard Molecules of Equivalent Fluoresein (MEFLs)
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