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Team:BostonU/Measurement
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| + | <td scope="col"><h2>Why is measurement important?</h2> | ||
| + | <br> | ||
| + | Synthetic Biology is a relatively new field of research. It involves the assembly of nucleic acid polymers in many possible arrangements and checking for the range of function that they exhibit. Thus, efficient measurement of genetic devices becomes integral to determining their function. <br><br> | ||
| + | <h2>Why is BU in the measurement track?</h2> | ||
| + | <br> | ||
| + | The BU Chimera workflow [link] efficiently combines software with wet lab protocols to yield complex devices. In using this workflow, one goes around the standard design-build-test cycles at least thrice, which are referred to as different phases [??]. Testing for any device involves the use of TASBE Tools and flow cytometry. This is used extensively in Phase 2 of the workflow as one multiplexing reaction can potentially yield many possible constructs. Here, measurement can be used to differentiate between a plethora of constructs and to pick the best combinatorial possibility for the assembly of the larger device. Measurement, hence, is an essential part of the Chimera workflow as the results from one phase are necessary as the inputs in the next phase. | ||
| + | <br><br> | ||
| + | <h2>Considerations in measurement strategy</h2> | ||
| + | <h3>Strength of E. Coli</h3> | ||
| + | <h3>Bioline Strain vs Pro Strain</h3> | ||
| + | <h3>Testing Strain?</h3> | ||
| + | <h3> Standardized Data for sharing and reproduction </h3> | ||
| + | </td> | ||
| + | </tr> | ||
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| + | </table> | ||
Revision as of 01:59, 16 October 2014
Why is measurement important?Synthetic Biology is a relatively new field of research. It involves the assembly of nucleic acid polymers in many possible arrangements and checking for the range of function that they exhibit. Thus, efficient measurement of genetic devices becomes integral to determining their function. Why is BU in the measurement track?The BU Chimera workflow [link] efficiently combines software with wet lab protocols to yield complex devices. In using this workflow, one goes around the standard design-build-test cycles at least thrice, which are referred to as different phases [??]. Testing for any device involves the use of TASBE Tools and flow cytometry. This is used extensively in Phase 2 of the workflow as one multiplexing reaction can potentially yield many possible constructs. Here, measurement can be used to differentiate between a plethora of constructs and to pick the best combinatorial possibility for the assembly of the larger device. Measurement, hence, is an essential part of the Chimera workflow as the results from one phase are necessary as the inputs in the next phase. Considerations in measurement strategyStrength of E. ColiBioline Strain vs Pro StrainTesting Strain?Standardized Data for sharing and reproduction |
