Team:BostonU/Chimera

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Project Chimera
Synthetic biology research revolves around design-build-test cycles for the production of genetic devices. An effective process often depends on protocol robustness and a thorough understanding of individual genetic components. Currently, limited software integration and part characterization represent significant stymying factors to the growth of the field, particularly as researchers endeavor to construct increasingly complex devices with behavior that is difficult to predict.

We seek to strengthen the traditional design-build-test cycle by developing a workflow that utilizes bio-design automation software tools and builds upon a thoroughly characterized library of parts. 		ChimeraPlasmid

The Chimera three-cycle workflow


The Chimera workflow is intended to facilitate the predictive design of complex genetic regulatory networks. It employs a three-cycle approach characterized by the use of computational tools: Eugene for designing, Raven for assembling, and the TASBE Tools for testing genetic constructs. Depending on the researcher's knowledge of device design and assembly, the Chimera workflow can be adjusted in its reliance on the computational tools employed.

workflow page, in which we test the functionality of Chimera by using it to assemble a priority encoder.

Phase I


Compile and test library of basic parts.

Phase II


Assemble and test range of TU variants.

Phase III


Assemble and test complex device.







Our Sponsors

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