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Team:Aix-Marseille/Parts
From 2014.igem.org
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Part BBa_K1349001
Name
RelA-2stop
Description
The relA gene encodes a ppGpp synthetase. In bacteria, ppGpp (guanosine 3'-diphosphate 5-' diphosphate) acts as a signaling molecule that regulates a variety of cellular metabolisms in response to changes in the nutritional state of the cells. The relA sequence amplified from E. coli W3110 was mutated by site-directed mutagenesis to remove the PstI site.
Part BBa_K1349002
Name
CusR box
Description
The CusR box allow the binding of the CusR regulator. In E. coli, the CusR/CusS two-component system induces expression of genes involved in metal efflux under condition of elevated Cu concentration. Phosphorylated CusR specifically bind to CusR-dependent promoters.
Other parts
Part BBa_K1349000 (SerA_mut)
Part BBa_K1349003 (P-cusR)
Part BBa_K1349004 (Mesh1-stop)
Part BBa_K1349005 ((SH3)x1)
Part BBa_K1349006 (CheA-stop)
Part BBa_K1349007 (CusR-LZA)
Part BBa_K1349008 ((SH3pep)-LZA)
