The project X-press: Characterization of pSBX-vectors made by Team Heidelberg
The iGEM team Heidelberg constructed a set of expression vectors that contain a T7 promoter prior to the BioBrick prefix. This way RBS-CDS parts can be cloned into the vectors by standard BioBrick assembly and then expressed in BL21(DE3) by induction with IPTG.
In collaboration with the iGEM Team Heidelberg the iGEM Team Aachen tested 8 of these vectors for their expression capabilities.
The Team Heidelberg expression vectors
These are the tested expression backbones:
| Plasmid | Backbone | Insert | Length vector | Length Insert | Resistance
| pSBX1A3-BBa_13507 (high copy) | pSBX1A3 | BBa_13507 | 3067 bp | 1226 bp | 50 mg/ml Ampicilin
|
| pSBX1C3-BBa_13507 (high copy) | pSBX1C3 | BBa_13507 | 2982 bp | 1226 bp | 35 mg/ml Chloramphenicol
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