Team:Bielefeld-CeBiTec/Notebook/Journal/Biosafety/Jun

From 2014.igem.org

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Design of the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#Integration_alr" target="_blank">Primer</a> for the integration of the konstitutive alanine racemase <i>alr</i> into the pSB1C3-Backbone.
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<li>Transformation of the <i>E. coli</i> strains KRX (<a href="https://www.promega.de/resources/protocols/technical-bulletins/101/single-step-krx-competent-cells-protocol/" target="_blank">Promega</a>) and DH5alpha (<a href="http://tools.lifetechnologies.com/content/sfs/manuals/11319019.pdf" target="_blank">Invitrogen</a>) with the plasmid pRedET containg the Recombinase using the <a href="http://www.genebridges.com/storage/Manuals_PDF/K006%20Ecoli%20Gene%20Deletion%20Kit-version2.3-2012.pdf" target="_blank">Genebridge RedET-System protocol</a>.
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Revision as of 11:29, 28 August 2014


June

Week 1     06/02 - 06/08

Week 1     06/02 - 06/08
  • Design of the Primer for the deletion of the whole coding sequence of the konstitutive alanine racemase (alr) and the katabolic alanine racemase (dadX) from E. coli using the Genebridge Red/ET-System.
    • Week 2     06/09 - 06/15

    Week 2     06/09 - 06/15
  • Design of the Primer for the integration of the konstitutive alanine racemase alr into the pSB1C3-Backbone.
    • Week 4     06/23 - 06/29

    Week 4     06/23 - 06/29
  • Transformation of the E. coli strains KRX (Promega) and DH5alpha (Invitrogen) with the plasmid pRedET containg the Recombinase using the Genebridge RedET-System protocol.

    • Retrieved from "http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Journal/Biosafety/Jun"