Team:Paris Saclay/Notebook

From 2014.igem.org

(Difference between revisions)
(C - Salicylate Inducible Suppressing System)
(C - Salicylate Inducible Suppressing System)
Line 70: Line 70:
*DNA extraction from gel agarose - ''7th August''
*DNA extraction from gel agarose - ''7th August''
*Ligation - ''7th August''
*Ligation - ''7th August''
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*Transformation in DH5 alpha - ''8th August''
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*Transformation of competent E.coli cells - ''8th August''
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*Bacterial Culture - ''8th August''
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*Liquid Culture - ''11th August''
*Liquid Culture - ''11th August''
*Plasmid DNA Purification - ''12th August''
*Plasmid DNA Purification - ''12th August''

Revision as of 17:57, 6 August 2014

Contents

Notebook

We constitute our team in November 2013 and we have meeting twice a month until April. Then we met every weeks. The Lab work started at the beginning of June.

Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work. Click here to edit this page

Planning

C - Salicylate Inducible Suppressing System

  • Rehydration of BioBricks BBa_J61051 and BBa_K228001- 23th July
  • Transformation of competent E.coli cells - 24th July
  • Bacterial culture - 25th July
  • Plasmid DNA Purification - 28th July
  • Digestion to check & Electrophoresis - 28th July
  • Main Digestion - 29th July
  • Segregate Process - 29th July
  • DNA extraction from gel agarose - 30th July
  • Ligation - 30th July
  • Transformation of competent E.coli cells - 31st July
  • Liquid Culture - 1st August
  • Plasmid DNA Purification - 4th August
  • Digestion to check & Electrophoresis - 4th August
  • Final Stock BBa_K1372000 - 4th August
  • Liquid Culture - 5th August
  • Plasmid DNA Purification - 6th August
  • Digestion of BBa_B0015 and BBa_K1372000 - 6th August
  • Segregate Process - 6th August
  • DNA extraction from gel agarose - 7th August
  • Ligation - 7th August
  • Transformation of competent E.coli cells - 8th August
  • Liquid Culture - 11th August
  • Plasmid DNA Purification - 12th August
  • Digestion to check & Electrophoresis - 12th August
  • Final Stock - 12th August

D - Lemon Scent

  • PCR of BBa_517003 and BBa_K762100 - 4th August
  • Gel electrophoresis of BBa_K517003 and BBa_K762100 - 5th August
  • PCR Cleanup of BBa_K517003 and BBa_K762100 - 6th August