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From 2014.igem.org

Revision as of 17:55, 6 August 2014 (view source) Maschi (Talk | contribs) (→C - Salicylate Inducible Suppressing System) ← Older edit		Revision as of 17:57, 6 August 2014 (view source) Maschi (Talk | contribs) (→C - Salicylate Inducible Suppressing System) Newer edit →
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	*DNA extraction from gel agarose - ''7th August''		*DNA extraction from gel agarose - ''7th August''
	*Ligation - ''7th August''		*Ligation - ''7th August''
-	*Transformation in DH5 alpha - ''8th August''	+	*Transformation of competent E.coli cells - ''8th August''
-	*Bacterial Culture - ''8th August''	+
	*Liquid Culture - ''11th August''		*Liquid Culture - ''11th August''
	*Plasmid DNA Purification - ''12th August''		*Plasmid DNA Purification - ''12th August''

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Revision as of 17:57, 6 August 2014

Contents 1 Notebook 1.1 Planning 1.1.1 C - Salicylate Inducible Suppressing System 1.1.2 D - Lemon Scent

Notebook

We constitute our team in November 2013 and we have meeting twice a month until April. Then we met every weeks. The Lab work started at the beginning of June.

Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work. Click here to edit this page

Planning

C - Salicylate Inducible Suppressing System

• Rehydration of BioBricks BBa_J61051 and BBa_K228001- 23th July
• Transformation of competent E.coli cells - 24th July
• Bacterial culture - 25th July
• Plasmid DNA Purification - 28th July
• Digestion to check & Electrophoresis - 28th July
• Main Digestion - 29th July
• Segregate Process - 29th July
• DNA extraction from gel agarose - 30th July
• Ligation - 30th July
• Transformation of competent E.coli cells - 31st July
• Liquid Culture - 1st August
• Plasmid DNA Purification - 4th August
• Digestion to check & Electrophoresis - 4th August
• Final Stock BBa_K1372000 - 4th August
• Liquid Culture - 5th August
• Plasmid DNA Purification - 6th August
• Digestion of BBa_B0015 and BBa_K1372000 - 6th August
• Segregate Process - 6th August
• DNA extraction from gel agarose - 7th August
• Ligation - 7th August
• Transformation of competent E.coli cells - 8th August
• Liquid Culture - 11th August
• Plasmid DNA Purification - 12th August
• Digestion to check & Electrophoresis - 12th August
• Final Stock - 12th August

D - Lemon Scent

• PCR of BBa_517003 and BBa_K762100 - 4th August
• Gel electrophoresis of BBa_K517003 and BBa_K762100 - 5th August
• PCR Cleanup of BBa_K517003 and BBa_K762100 - 6th August