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Team:UCL/Science/Bioprocessing/Flowsheet
From 2014.igem.org
with azodyes
The contamination of natural habitats surrounding textile factories by coloured (azodye-rich) effluents is a real problem (more). This is because the enzymatic breakdown products of azodyes i.e. aromatic amines, are carcinogenic when ingested. These can not only build up within local ecosystems but can also be a hazard to humans through bio-accumulation in the food chain. With a large section of dyehouse effluents consisting of dyes that have half-lives spanning over decades, the latter remain in the environment for long periods of time.
Understanding the issues
with current methods
With regards to current technologies in the textile industry, exorbitant volumes of water are used for processing (around 90%), the rest being used for heat exchange purposes. Unfortunately most of the water used for processing is discharged as waste, resulting in highly diluted azodye effluent streams. Secondly, the recalcitrant nature of azodyes hikes the inherent costs of large-scale physical separation systems. As a result, industrial processes used to deal with such soluble hazardous wastes would not be a feasible option to deal with azodye effluents.
Whole-cell biocat
By using whole cell biocatalysis as the workhorse for detoxification, this process will yield lucrative byproducts such as quinones, that can then be separated from the process stream and sold off.
Our Solution
Overview of the solution
Concentration
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Dyestuff industry
By considering the production of azodyes within a specific sector, it is possible to approximate a yearly production of azodyes, suited a given purpose. In this scenario, we are looking at the dyeing process of 1kg of cotton using methyl red.
Process inputs
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Reaction kinetics
Several assumptions need to be made in order to determine the basic kinetic rates for our system.
Immobilization methods
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Biofilms
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Whole-cells vs. Enzymes
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Current methods
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Other Problems
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Other Problems
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Other Problems
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Other Problems
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Other Problems
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Other Problems
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