Team:UESTC-China

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<div id="header">
<div id="header">
<div id="emp">
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<div id='logo'><a href="https://igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2014/6/60/LiLogo.png"></a></div>
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<a href="https://2014.igem.org/Team:UESTC-China/Design"><li>Design</li></a>
<a href="https://2014.igem.org/Team:UESTC-China/Design"><li>Design</li></a>
<a href="https://2014.igem.org/Team:UESTC-China/result"><li>Result</li></a>
<a href="https://2014.igem.org/Team:UESTC-China/result"><li>Result</li></a>
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<a href="https://2014.igem.org/Team:UESTC-China/Futurework">
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<a href="https://2014.igem.org/Team:UESTC-China/Futurework"><li>Future work</li></a>
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<li>Future work</li></a>
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<a href="https://2014.igem.org/Team:UESTC-China/Judingform"><li>Judging Criteria</li></a>
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<a href="https://2014.igem.org/Team:UESTC-China/Modeling"><li>Modeling</li></a>
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<a href="https://2014.igem.org/Team:UESTC-China/Modeling"><li>Overview</li></a>
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<a href="https://2014.igem.org/Team:UESTC-China/Modeling1"><li>HPS/PHI</li></a>
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<a href="https://2014.igem.org/Team:UESTC-China/Modeling2"><li>FALDH/FDH</li></a>
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<a href="https://2014.igem.org/Team:UESTC-China/Modeling3"><li>AtAHA2</a>
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<a href="https://2014.igem.org/Team:UESTC-China/Material"><li>BioBrick</li></a>
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<a href="https://2014.igem.org/Team:UESTC-China/BioBrick"><li>BioBrick</li></a>
<a href="https://2014.igem.org/Team:UESTC-China/Protocol"><li>Protocol</li></a>
<a href="https://2014.igem.org/Team:UESTC-China/Protocol"><li>Protocol</li></a>
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<div class="top-menu-sub">
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<ul>
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<a href="https://2014.igem.org/Team:UESTC-China/HumanPractice.html"><li>Knowledge quiz</li></a>
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<a href="https://2014.igem.org/Team:UESTC-China/Questionnaire.html"><li>Questionnaire</li></a>
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<a href="https://2014.igem.org/Team:UESTC-China/Knowledgequiz"><li>Knowledge quiz</li></a>
<a href="https://2014.igem.org/Team:UESTC-China/Lecture.html"><li>Lecture</li></a>
<a href="https://2014.igem.org/Team:UESTC-China/Lecture.html"><li>Lecture</li></a>
<a href="https://2014.igem.org/Team:UESTC-China/Communication.html"><li>Communication</li></a>
<a href="https://2014.igem.org/Team:UESTC-China/Communication.html"><li>Communication</li></a>
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<a href="https://2014.igem.org/Team:UESTC-China/Questionnaire.html"><li>Questionnaire</li></a>
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<a href="https://2014.igem.org/Team:UESTC-China/Art"><li>Art</li></a>
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<div><img src="https://static.igem.org/mediawiki/2014/1/15/NewPlant1.jpg"></div>
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<p class="header">Our Team</p>
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<div><img src="https://static.igem.org/mediawiki/2014/8/83/Home1.jpg"></div>
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<p class="content">Brilliant guys.</p>
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<div><img src="https://static.igem.org/mediawiki/2014/8/8b/Xiaojiejie.jpg"></div>
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                <div><img src="https://static.igem.org/mediawiki/2014/c/ce/Humanpractice.jpg"></div>
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    <p class="active" style="bottom: 0px;"><span>Our Team: Brilliant Guys</span></p>
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        <p style="bottom: -40px;">Plants: Transgenic Tobaccos</p>
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        <p style="bottom: -40px;">Super Plant: Plants vs HCOH</p>
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        <p style="bottom: -40px;">Safety: Operation</p>
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        <p style="bottom: -40px;">Human Practice</p>
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<span class="active" rel="1">1</span>
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<span rel="2" class="">2</span>
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<span rel="3" class="">3</span>
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<span rel="4" class="">4</span>
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        <span rel="5" class="">5</span>
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<div id="introduction">
<div id="introduction">
<p id="intro1"><b>Introduction</b></p>
<p id="intro1"><b>Introduction</b></p>
<br/>
<br/>
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<p id="intro2">As the rapid economic development in China, the residential conditions of people have been greatly improved. Unfortunately, the potential harm of their indoor decoration was always neglected until people get sick. One of the most harmful gases of the indoor pollution could be the formaldehyde.<br/>Formaldehyde is a toxic gas appears in every single room which is newly decorated. It not only has bad smell, but also cause various of desease, like skin and mucous membrane irritation, immunity or memory decline, drowsiness, fatigue, cancer, allergic dermatitis.<br/>To decrease the concentration of HCHO, we got air purifier, physical adsorption, negative air ions, photocatalyst pathway and etc. We thought that the biological methods is the best. It’s delicate, it’s simple, and it’s safe. Therefore, we decided to cultivate a plant which got the ability to metabolize the formaldehyde. Actually, all kinds of plants can degrade the formaldehyde, but we decide to make their formaldehyde degradation system works better.<br/>In the future,we sincerely chrish the hope that our work will be put to use in our everyday life.</p>
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<p id="intro2" style="color:white;text-indent: 0em">
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<br/>
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With the rapid society development, a large amount of pollutants are full of our daily life. Nowadays, chemical materials are widely used in our surroundings indoor, such as building materials, paint in new apartments, artificial adhesives in numerous household products and etc, which may lead to excess release of formaldehyde in closed space. Exposing to the substantial formaldehyde may cause irritation, allergic asthma and neurasthenia, as well as carcinogenesis. Nowadays, people mainly get rid of the formaldehyde by increasing the rate of ventilating or using air purifier in those polluted spaces. However, these methods show some limitation, and cannot degrade formaldehyde safely and effectively.
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<img width="1215px" src="https://static.igem.org/mediawiki/2010/9/9c/Theory_Home-foot_UNR.png" alt="pic">
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<br/><br/>
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To get the inspiration for the design of our project, we did a survey of the knowledge concerning formaldehyde pollution. The results showed that people paid high attention to the indoor formaldehyde pollution, and preferred to adopt a safe and environmental friendly method to decrease the concentration of formaldehyde effectively. A methodology by using green plants got the highest score among all provided approaches. Based on the results of this survey, we decided to use synthetic biology strategy to design a super plant that can remove formaldehyde safely and effectively. 
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<br/><br/>
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After doing sufficient literature research, we got to know that there is a formaldehyde-fixation pathway in methylotrophs which is called ribulose monophosphate (RuMP) pathway. HPS and PHI are the two key enzymes in this pathway. Besides, there is a formaldehyde folate-independent pathway in some certain plant species. FALDH and FDH play an important role in this pathway. In our 2014 iGEM project, these two pathways were integrated through the methodology of synthetic biology. For this, formaldehyde metabolism related genes from both methylotrophs and plants were transferred into tobacco to rebuild a new formaldehyde metabolism pathway in our super plant.
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<br/><br/>
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To further increase the degradation efficiency of formaldehyde, we co-expressed gene <i>AHA2</i>, which is original from <i>Arabidopsis thaliana</i>, to regulate stomata opening. In addition, by taking into account of biosafety of genetically modified organism, we introduced the gene of cysteine protease into our super plants. The transgenic tobacco pollen will be aborted while cysteine protease gene is specifically expressed in tobacco tapetum.
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<br/><br/>
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With more and more achievements have been harvested about the super plant, we organized various activities such as questionnaire survey, school seminar series, video making, etc, to vigorously promote synthetic biology, iGEM and our super plant. Along with the happy summer vacation, we had completed our project successfully with the efforts of all members. Let's meet at MIT and share each other's achievenments.
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<br/><br/>
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<img style="height:300px;width:1215px" src="https://static.igem.org/mediawiki/2014/8/89/Jiantu5.jpg" alt="pic">
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</p>
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<p><b>Contact us:</b>cys@gmail.com</p>
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Latest revision as of 15:14, 17 October 2014

Plant Vs HCHO

Our Team: Brilliant Guys

Plants: Transgenic Tobaccos

Super Plant: Plants vs HCOH

Safety: Operation

Human Practice

1 2 3 4 5

Introduction


With the rapid society development, a large amount of pollutants are full of our daily life. Nowadays, chemical materials are widely used in our surroundings indoor, such as building materials, paint in new apartments, artificial adhesives in numerous household products and etc, which may lead to excess release of formaldehyde in closed space. Exposing to the substantial formaldehyde may cause irritation, allergic asthma and neurasthenia, as well as carcinogenesis. Nowadays, people mainly get rid of the formaldehyde by increasing the rate of ventilating or using air purifier in those polluted spaces. However, these methods show some limitation, and cannot degrade formaldehyde safely and effectively.

To get the inspiration for the design of our project, we did a survey of the knowledge concerning formaldehyde pollution. The results showed that people paid high attention to the indoor formaldehyde pollution, and preferred to adopt a safe and environmental friendly method to decrease the concentration of formaldehyde effectively. A methodology by using green plants got the highest score among all provided approaches. Based on the results of this survey, we decided to use synthetic biology strategy to design a super plant that can remove formaldehyde safely and effectively.

After doing sufficient literature research, we got to know that there is a formaldehyde-fixation pathway in methylotrophs which is called ribulose monophosphate (RuMP) pathway. HPS and PHI are the two key enzymes in this pathway. Besides, there is a formaldehyde folate-independent pathway in some certain plant species. FALDH and FDH play an important role in this pathway. In our 2014 iGEM project, these two pathways were integrated through the methodology of synthetic biology. For this, formaldehyde metabolism related genes from both methylotrophs and plants were transferred into tobacco to rebuild a new formaldehyde metabolism pathway in our super plant.

To further increase the degradation efficiency of formaldehyde, we co-expressed gene AHA2, which is original from Arabidopsis thaliana, to regulate stomata opening. In addition, by taking into account of biosafety of genetically modified organism, we introduced the gene of cysteine protease into our super plants. The transgenic tobacco pollen will be aborted while cysteine protease gene is specifically expressed in tobacco tapetum.

With more and more achievements have been harvested about the super plant, we organized various activities such as questionnaire survey, school seminar series, video making, etc, to vigorously promote synthetic biology, iGEM and our super plant. Along with the happy summer vacation, we had completed our project successfully with the efforts of all members. Let's meet at MIT and share each other's achievenments.

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