Team:The Tech Museum/Notebook

From 2014.igem.org

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<p>You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well. </p>
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<p>Timeline of major events:<br>
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May: Basic concept research and design<br>
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June: Software development initiated; plasmid design started and promoter-RBS’s picked<br>
 +
July: Wetlab testing of possible color protein combinations<br>
 +
August 10: Design of complete tri-color plasmids finalized<br>
 +
August 24: Assembly of tri-color plasmid pools completed (DNA2.0)<br>
 +
September 10: Tri-color plasmids optimized on museum wetlab setup<br>
 +
September 29: Software fully integrated with hardware on mobile exhibit<br>
 +
September 30 - October 3: Prototyping of mobile exhibit with museum staff and visitors<br>
 +
October 6 - October 17: Data collection on the museum floor with visitor!</p>
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<p><b>Biology Details</b></p>
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<p>Design of tri-color plasmid pool (June 2014)<br>
 +
9 promoter-rbs pairs of varying strength from Kosuri et al paper (2013):<br>
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<table border="1" cellpadding="0" cellspacing="0">
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<td></td>
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<td>Promoter - RBS Pairs</td>
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<td>Sequence</td>
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</tr>
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<tr>
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<td></td>
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<td>Promoter - RBS Pairs</td>
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<td>Sequence</td>
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</tr>
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<tr>
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<td></td>
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<td>Promoter - RBS Pairs</td>
 +
<td>Sequence</td>
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</tr>
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<tr>
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<td></td>
 +
<td>Promoter - RBS Pairs</td>
 +
<td>Sequence</td>
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</tr>
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<tr>
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<td></td>
 +
<td>Promoter - RBS Pairs</td>
 +
<td>Sequence</td>
 +
</tr>
 +
<tr>
 +
<td></td>
 +
<td>Promoter - RBS Pairs</td>
 +
<td>Sequence</td>
 +
</tr>
 +
<tr>
 +
<td></td>
 +
<td>Promoter - RBS Pairs</td>
 +
<td>Sequence</td>
 +
</tr>
 +
<tr>
 +
<td></td>
 +
<td>Promoter - RBS Pairs</td>
 +
<td>Sequence</td>
 +
</tr>
 +
<tr>
 +
<td></td>
 +
<td>Promoter - RBS Pairs</td>
 +
<td>Sequence</td>
 +
</tr>
 +
<tr>
 +
<td></td>
 +
<td>Promoter - RBS Pairs</td>
 +
<td>Sequence</td>
 +
</tr>
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</table>
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</td>
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Revision as of 18:34, 16 October 2014

Home Team Project Notebook Community Engagement Attributions

Notebook

Timeline of major events:
May: Basic concept research and design
June: Software development initiated; plasmid design started and promoter-RBS’s picked
July: Wetlab testing of possible color protein combinations
August 10: Design of complete tri-color plasmids finalized
August 24: Assembly of tri-color plasmid pools completed (DNA2.0)
September 10: Tri-color plasmids optimized on museum wetlab setup
September 29: Software fully integrated with hardware on mobile exhibit
September 30 - October 3: Prototyping of mobile exhibit with museum staff and visitors
October 6 - October 17: Data collection on the museum floor with visitor!

Biology Details

Design of tri-color plasmid pool (June 2014)
9 promoter-rbs pairs of varying strength from Kosuri et al paper (2013):

Promoter - RBS Pairs Sequence
Promoter - RBS Pairs Sequence
Promoter - RBS Pairs Sequence
Promoter - RBS Pairs Sequence
Promoter - RBS Pairs Sequence
Promoter - RBS Pairs Sequence
Promoter - RBS Pairs Sequence
Promoter - RBS Pairs Sequence
Promoter - RBS Pairs Sequence
Promoter - RBS Pairs Sequence