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Team:Saarland/Test
From 2014.igem.org
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| - | The following speed cycle program was used:<br> <br> | + | [[File:Checkmark-small.png|left]]The following speed cycle program was used:<br> <br> |
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Revision as of 19:50, 15 October 2014
| Step | Temperature [°C] | Time [s] |
|---|---|---|
| 1. Initial Denaturation | 98 | 30 |
| 2. Denaturation | 98 | 15 |
| 3. Annealing | 60 | 15 |
| 4. Extention | 75 | 20/kb |
| 5. Final Extention | 72 | 120 |
| 6. storage | 4 | for ever |
30 cycles
Impressum/Copyright