Team:Saarland/Test

From 2014.igem.org

(Difference between revisions)
Line 42: Line 42:
<div style="margin-top:-140px; margin-left:625px">
<div style="margin-top:-140px; margin-left:625px">
<img src="https://static.igem.org/mediawiki/2014/6/6c/Pfeil_01.png">
<img src="https://static.igem.org/mediawiki/2014/6/6c/Pfeil_01.png">
-
<p> 30 cycles</p>
+
30 cycles
</div>
</div>
</html>
</html>

Revision as of 14:17, 15 October 2014


The following speed cycle program was used:

Step Temperature [°C] Time [s]
1. Initial Denaturation 98 30
2. Denaturation 98 15
3. Annealing 60 15
4. Extention 75 20/kb
5. Final Extention 72 120
6. storage 4 for ever

30 cycles



Retrieved from "http://2014.igem.org/Team:Saarland/Test"