Team:Saarland/Test

From 2014.igem.org

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The following speed cycle program was used:<br> <br>
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<!-- TAbelle -->  <!--Pfeil!!!! --->
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{|
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|+
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!Step
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!Temperature [°C]
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!Time [s]
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|-
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|1. Initial Denaturation
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|98
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| 30
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|-
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| 2. Denaturation
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|98
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|15
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|-
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|3. Annealing
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|60
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|15
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|-
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|4. Extention
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|75
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|20/kb
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|-
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|5. Final  Extention
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|72
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|120
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|-
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|6. storage
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|4
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|for ever
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|-
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|}
<html>
<html>
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<div style=" margin-top:100px; margin-left:600px">
<img src="https://static.igem.org/mediawiki/2014/6/6c/Pfeil_01.png">
<img src="https://static.igem.org/mediawiki/2014/6/6c/Pfeil_01.png">
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<p> 30 cycles</p>
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</html>

Revision as of 14:15, 15 October 2014


The following speed cycle program was used:

Step Temperature [°C] Time [s]
1. Initial Denaturation 98 30
2. Denaturation 98 15
3. Annealing 60 15
4. Extention 75 20/kb
5. Final Extention 72 120
6. storage 4 for ever

30 cycles



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