Team:SZU-China

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          <div class="slide-text "> Alkaline cellulase Deinking </div>
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        <h3> <b>Super Dream Factory of Cellulase </b></h3>
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      <br>
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      <br>
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      <figure class="thumbnail zan-thumb"><img width="450" height="240" src="https://static.igem.org/mediawiki/2014/2/28/SzuHome33.jpg"  class="lazy attachment-medium wp-post-image" alt="banner_7" /> </figure>
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      <p style="font-size:large">We have made a super bacteria-Super Dream Factory of Cellulase.The factory has four parts. They are alkaline cellulase, Mdfa antiporter, kil &nbsp; protein and ccdB suicide system. <br>
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        To enhance the effects of decontamination in various fields, we use an alkaline cellulase as detergent additive. The alkaline cellulase gene was introduced into the Escherichia coli and the target enzyme can be highly produced with the help of Kil, which is one of the core elements of our project. What’s more, MdfA, a proton pump across the cell membrane, was added to transport the proton into the cytoplasma, and release Na<sup>+</sup>/K<sup>+</sup> at the same time, resulting in cell survival in alkaline industry wastewaters as high as pH 9.5. Another highlight of our project is that we also added a ccdB suicide system to our e-machine, by this we could remove the biological machine whenever we need for biological safety. </p>
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  <div class="pull-right post-info"> <span><i class="fa fa-comment"></i> From SZU-China</span> </div>
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      ©2014 SZU-China
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
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<a href="https://2014.igem.org/Team:SZU-China"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:SZU-China/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=SZU-China"style="color:#000000"> Official Team Profile </a></td>
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<td style="border:1px solid black" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
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<a href="https://2014.igem.org/Team:SZU-China/Project"style="color:#000000"> Project</a></td>
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<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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<a href="https://2014.igem.org/Team:SZU-China/Parts"style="color:#000000"> Parts</a></td>
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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<a href="https://2014.igem.org/Team:SZU-China/Modeling"style="color:#000000"> Modeling</a></td>
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
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<a href="https://2014.igem.org/Team:SZU-China/Notebook"style="color:#000000"> Notebook</a></td>
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<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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<a href="https://2014.igem.org/Team:SZU-China/Safety"style=" color:#000000"> Safety </a></td>
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<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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<a href="https://2014.igem.org/Team:SZU-China/Attributions"style="color:#000000"> Attributions </a></td>
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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<!--requirements section -->
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<tr><td colspan="3"> <h3> Requirements </h3></td></tr>
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<p> Please be sure to keep these links, your audience will want to find your: </p>
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<!-- Links to other team pages -->
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<ul>
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<li><a href="https://2014.igem.org/Team:SZU-China">Home</a> </li>
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<li><a href="https://2014.igem.org/Team:SZU-China/Team">Team</a> </li>
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=SZU-China">Official Team Profile</a> </li>
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<li><a href="https://2014.igem.org/Team:SZU-China/Project">Project</a> </li>
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<li><a href="https://2014.igem.org/Team:SZU-China/Parts">Parts</a> </li>
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<li><a href="https://2014.igem.org/Team:SZU-China/Modeling">Modeling</a> </li>
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<li><a href="https://2014.igem.org/Team:SZU-China/Notebook">Notebook</a> </li>
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<li><a href="https://2014.igem.org/Team:SZU-China/Safety">Safety</a> </li>
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<li><a href="https://2014.igem.org/Team:SZU-China/Attributions">Attributions</a> </li>
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</ul>
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<td > </td>
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<p>There are a few wiki requirements teams must follow:</p>
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<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
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<li>All pages must be created under the team’s name space.</li>
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<li>As part of your documentation, keep the links from the menu to the left. </li>
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<li>Do not use flash in wiki code. </li>
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<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
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</ul>
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<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
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<tr> <td colspan="3"  height="15px"> </td></tr>
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<!--tips  -->
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<tr><td colspan="3" > <h3> Tips  </h3></td></tr>
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<p>We are currently working on providing teams with some easy to use design templates.
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
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<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
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<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
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<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
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</ul>
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
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</td>
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<td> </td>
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<td width="45%">
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<ul>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and what you plan to do.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
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<li>Have lots of fun! </li>
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</ul>
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</br>
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</td>
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</table>
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Latest revision as of 02:29, 18 October 2014

SZU-China


Super Dream Factory of Cellulase



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We have made a super bacteria-Super Dream Factory of Cellulase.The factory has four parts. They are alkaline cellulase, Mdfa antiporter, kil   protein and ccdB suicide system.
To enhance the effects of decontamination in various fields, we use an alkaline cellulase as detergent additive. The alkaline cellulase gene was introduced into the Escherichia coli and the target enzyme can be highly produced with the help of Kil, which is one of the core elements of our project. What’s more, MdfA, a proton pump across the cell membrane, was added to transport the proton into the cytoplasma, and release Na+/K+ at the same time, resulting in cell survival in alkaline industry wastewaters as high as pH 9.5. Another highlight of our project is that we also added a ccdB suicide system to our e-machine, by this we could remove the biological machine whenever we need for biological safety.



Retrieved from "http://2014.igem.org/Team:SZU-China"