Team:SZU-China

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           <li id="menu-item-215" class="menu-item menu-item-type-custom menu-item-object-custom current-menu-item current_page_item menu-item-home menu-item-215"><a title="Home" href="https://2014.igem.org/Team:SZU-China"><i class="fa fa-home"></i> Home</a></li>
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           <li id="menu-item-895" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-has-children menu-item-895"><a title="Project" ><i class=" fa fa-edit"></i> Project</a>
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               <li id="menu-item-896" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-896"><a href="https://2014.igem.org/Team:SZU-China/Project/background">background</a></li>
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               <li id="menu-item-897" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-897"><a href="https://2014.igem.org/Team:SZU-China/Project/overview">Overview</a></li>
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               <li id="menu-item-897" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-897"><a href="https://2014.igem.org/Team:SZU-China/Project/overview">overview</a></li>
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               <li id="menu-item-896" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-896"><a href="https://2014.igem.org/Team:SZU-China/Project/AlkalineCellulase">Alkaline Cellulase</a></li>
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               <li id="menu-item-898" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-898"><a href="https://2014.igem.org/Team:SZU-China/Project/design">design</a></li>
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               <li id="menu-item-898" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-898"><a href="https://2014.igem.org/Team:SZU-China/Project/Mdfa">Mdfa Antiporter</a></li>
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               <li id="menu-item-899" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-899"><a href="https://2014.igem.org/Team:SZU-China/Project/result">result</a></li>
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               <li id="menu-item-899" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-899"><a href="https://2014.igem.org/Team:SZU-China/Project/Kil">Kil Protein</a></li>
 +
              <li id="menu-item-900" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-900"><a href="https://2014.igem.org/Team:SZU-China/Project/ccdB">ccdB Suicide System</a></li>
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              <li id="menu-item-901" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-901"><a href="https://2014.igem.org/Team:SZU-China/Project/Collaboration">Collaboration</a></li>
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               <li id="menu-item-997" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-997"><a href="https://2014.igem.org/Team:SZU-China/Team/member">member</a></li>
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               <li id="menu-item-996" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-996"><a href="https://2014.igem.org/Team:SZU-China/Team/Attribution">Attribution</a></li>
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               <li id="menu-item-939" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-939"><a href="https://igem.org/Team.cgi?year=2014">official team profile</a></li>
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              <li id="menu-item-940" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-940"><a href="https://2014.igem.org/Team:SZU-China/Team/Acknowledgment">Acknowledgment</a></li>
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               <li id="menu-item-939" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-939"><a href="https://igem.org/Team.cgi?year=2014">Official team profile</a></li>
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           <li id="menu-item-676" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-has-children menu-item-676"><a><i class="fa fa-globe"></i> Human Practice</a>
           <li id="menu-item-676" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-has-children menu-item-676"><a><i class="fa fa-globe"></i> Human Practice</a>
             <ul class="dropdown-menu">
             <ul class="dropdown-menu">
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              <li id="menu-item-676" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-676"><a target="_blank" href="https://2014.igem.org/Team:SZU-China/HumanPractice/Overview">Overview</a></li>
               <li id="menu-item-677" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-677"><a target="_blank" href="https://2014.igem.org/Team:SZU-China/HumanPractice/CulturalFestival">Cultural Festival</a></li>
               <li id="menu-item-677" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-677"><a target="_blank" href="https://2014.igem.org/Team:SZU-China/HumanPractice/CulturalFestival">Cultural Festival</a></li>
               <li id="menu-item-678" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-678"><a target="_blank" href="https://2014.igem.org/Team:SZU-China/HumanPractice/Communication">Communication</a></li>
               <li id="menu-item-678" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-678"><a target="_blank" href="https://2014.igem.org/Team:SZU-China/HumanPractice/Communication">Communication</a></li>
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               <li id="menu-item-680" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-680"><a target="_blank" href="https://2014.igem.org/Team:SZU-China/HumanPractice/PaperMill">PaperMill</a></li>
+
               <li id="menu-item-680" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-680"><a target="_blank" href="https://2014.igem.org/Team:SZU-China/HumanPractice/PaperMill">Paper Mill</a></li>
 +
              <li id="menu-item-680" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-680"><a target="_blank" href="https://2014.igem.org/Team:SZU-China/HumanPractice/Survey">Survey</a></li>
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           </li>
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           <li id="menu-item-607" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-607"><a title="Judging"><i class="fa fa-check"></i> Judging</a>
           <li id="menu-item-607" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-607"><a title="Judging"><i class="fa fa-check"></i> Judging</a>
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+
               <li class="menu-item menu-item-type-custom menu-item-object-custom"> <a target="_blank" href="https://2014.igem.org/Team:SZU-China/judging/achievement">Achievement</a></li>
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               <li class="menu-item menu-item-type-custom menu-item-object-custom"> <a target="_blank" href="https://2014.igem.org/Team:SZU-China/judging/biobrick">biobrick</a></li>
+
               <li class="menu-item menu-item-type-custom menu-item-object-custom"> <a target="_blank" href="https://2014.igem.org/Team:SZU-China/judging/biobrick">Biobrick</a></li>
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               <li class="menu-item menu-item-type-custom menu-item-object-custom"> <a target="_blank" href="https://2014.igem.org/Team:SZU-China/Safety">safety</a></li>
+
               <li class="menu-item menu-item-type-custom menu-item-object-custom"> <a target="_blank" href="https://2014.igem.org/Team:SZU-China/Safety">Safety</a></li>
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           <li id="menu-item-1070" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-1070"><a href="https://2014.igem.org/Team:SZU-China/notebook"><i class="fa fa-book"></i>NoteBook</a></li>
           <li id="menu-item-1070" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-1070"><a href="https://2014.igem.org/Team:SZU-China/notebook"><i class="fa fa-book"></i>NoteBook</a></li>
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    <div class="flexslider <input type="email">
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          <div class="slide-text "> Cultural Festival & Practice </div>
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        <div class="slide-text hidden-xs"> Cultural Festival </div>
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          <div class="slide-text">Communication</div>
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          <div class="slide-text ">Paper Mill HP</div>
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        <div class="slide-text hidden-xs">Cultural Festival</div>
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          <div class="slide-text "> Experiment Results </div>
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    <div class="col-md-7 visible-lg bg-info 微软雅黑"> To enhance the effects of decontamination in various fields, we use an alkaline cellulase as detergent additive. The alkaline cellulase gene was introduced into the Escherichia coli and the target enzyme can be highly produced with the help of Kil, which is one of the core elements of our project. What’s more, MdfA, a proton pump across the cell membrane, was added to transport the proton into the cytoplasma, and release Na+/K+ at the same time, resulting in cell survival in alkaline industry wastewaters as high as pH 10. Another highlight of our project is that we also added a ccdAB suicide system to our e-machine, by this we could remove the biological machine whenever we need for biological safety. <br>
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      <p style="font-size:large">We have made a super bacteria-Super Dream Factory of Cellulase.The factory has four parts. They are alkaline cellulase, Mdfa antiporter, kil &nbsp; protein and ccdB suicide system. <br>
 +
        To enhance the effects of decontamination in various fields, we use an alkaline cellulase as detergent additive. The alkaline cellulase gene was introduced into the Escherichia coli and the target enzyme can be highly produced with the help of Kil, which is one of the core elements of our project. What’s more, MdfA, a proton pump across the cell membrane, was added to transport the proton into the cytoplasma, and release Na<sup>+</sup>/K<sup>+</sup> at the same time, resulting in cell survival in alkaline industry wastewaters as high as pH 9.5. Another highlight of our project is that we also added a ccdB suicide system to our e-machine, by this we could remove the biological machine whenever we need for biological safety. </p>
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Latest revision as of 02:29, 18 October 2014

SZU-China

What we do

Super Dream Factory of Cellulase



banner_7

We have made a super bacteria-Super Dream Factory of Cellulase.The factory has four parts. They are alkaline cellulase, Mdfa antiporter, kil   protein and ccdB suicide system.
To enhance the effects of decontamination in various fields, we use an alkaline cellulase as detergent additive. The alkaline cellulase gene was introduced into the Escherichia coli and the target enzyme can be highly produced with the help of Kil, which is one of the core elements of our project. What’s more, MdfA, a proton pump across the cell membrane, was added to transport the proton into the cytoplasma, and release Na+/K+ at the same time, resulting in cell survival in alkaline industry wastewaters as high as pH 9.5. Another highlight of our project is that we also added a ccdB suicide system to our e-machine, by this we could remove the biological machine whenever we need for biological safety.


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