Team:Oxford/Results
From 2014.igem.org
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<img src="https://static.igem.org/mediawiki/2014/7/7f/Oxford_charac3.png" style="float:right;position:relative; width:80%; margin-right:10%;margin-bottom:2%;margin-left:10%;" /><br> | <img src="https://static.igem.org/mediawiki/2014/7/7f/Oxford_charac3.png" style="float:right;position:relative; width:80%; margin-right:10%;margin-bottom:2%;margin-left:10%;" /><br> | ||
- | <strong>Figure 1</strong> | + | <strong>Figure 1</strong>shows the the gene region under investigation and manipulation in the design of our biosensor. PdcmA and PdcmR describe the two directions of a bidirectional promoter that is bound and modulated by DcmR |
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<img src="https://static.igem.org/mediawiki/2014/b/b7/Oxford_CharacGlen1.png" style="float:left;position:relative; width:80%;margin-bottom:2%;margin-left:10%;margin-right:10%;" /> | <img src="https://static.igem.org/mediawiki/2014/b/b7/Oxford_CharacGlen1.png" style="float:left;position:relative; width:80%;margin-bottom:2%;margin-left:10%;margin-right:10%;" /> | ||
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- | <strong>Figure 2</strong> | + | <strong>Figure 2</strong> shows the effect of adding DcmR to the system (through addition of plasmid POXON-2). Timecourse fluorescence data normalised to OD (600nm) is shown and summarised in the bar charts. Data is shown for both promoter directions (PdcmA and PdcmR). Standard error bars are shown. |
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Conclusion: DcmR is a repressor of both the PdcmA and PdcmR promoter. | Conclusion: DcmR is a repressor of both the PdcmA and PdcmR promoter. | ||
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<img src="https://static.igem.org/mediawiki/2014/1/18/Oxford_CharacGlen2.png" style="float:right;position:relative; width:80%; margin-right:10%;margin-bottom:2%;margin-left:10%;" /> | <img src="https://static.igem.org/mediawiki/2014/1/18/Oxford_CharacGlen2.png" style="float:right;position:relative; width:80%; margin-right:10%;margin-bottom:2%;margin-left:10%;" /> | ||
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- | <strong>Figure 3</strong> | + | <strong>Figure 3</strong> shows an OD(600nm) growth curve for all transformed bacteria investigated. The effect of DcmR is measured on the relative stoichiometry of activation of PdcmA/PdcmR. This is shows in the bar charts for stationary phase and for exponential phase. The cut of for exponential phase was set at 600 minutes. |
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<img src="https://static.igem.org/mediawiki/2014/2/2c/Oxford_CharacGlen4.png" style="float:right;position:relative; width:80%; margin-right:10%;margin-bottom:2%;margin-left:10%;" /> | <img src="https://static.igem.org/mediawiki/2014/2/2c/Oxford_CharacGlen4.png" style="float:right;position:relative; width:80%; margin-right:10%;margin-bottom:2%;margin-left:10%;" /> | ||
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- | <strong>Figure 4</strong> | + | <strong>Figure 4</strong> shows the effect of DCM on all both promoters both alone and under the influence of DcmR. Fluorescence measurements were made at mid-log phase with 30mM DCM where added. Yellow charts show the effect of DCM on expression downstream of the PdcmA promoter. Blue charts show the effect of DCM on expression downstream of the PdcmR promoter. Fluorescence measurements were normalised for OD (600nm). |
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From these results we can see that in the direction of PdcmA DCM has a modulating effect of relieving DcmR repression thus DCM acts through depression. Interestingly, DCM appears to decrease sfGFP fluorescence in the absence of DcmR. | From these results we can see that in the direction of PdcmA DCM has a modulating effect of relieving DcmR repression thus DCM acts through depression. Interestingly, DCM appears to decrease sfGFP fluorescence in the absence of DcmR. |
Revision as of 02:16, 18 October 2014