Team:Oxford

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<div class="inner" style="width:100%;margin-left:auto;margin-right:auto;height:100%;position:absolute;">
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<img class="DCMationgif" style="margin-left:5%;" src="https://static.igem.org/mediawiki/2014/8/81/DCMation1.png" width="90%" align="middle" alt="" onclick='javascript:(this.src=="https://static.igem.org/mediawiki/2014/8/81/DCMation1.png"?this.src="https://static.igem.org/mediawiki/2014/e/e2/OxigemDCMationgif.gif":"")' />
<img class="DCMationgif" style="margin-left:5%;" src="https://static.igem.org/mediawiki/2014/8/81/DCMation1.png" width="90%" align="middle" alt="" onclick='javascript:(this.src=="https://static.igem.org/mediawiki/2014/8/81/DCMation1.png"?this.src="https://static.igem.org/mediawiki/2014/e/e2/OxigemDCMationgif.gif":"")' />
 +
University of Oxford’s first iGEM team presents: DCMation, a novel bioremediation approach whose applications are limited only by the versatility of bacterial metabolism. OxiGEM are tackling environmental pollution by developing a user-friendly device for the detection & degradation of the hazardous yet indispensable solvent dichloromethane (DCM), to illustrate.
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<br>
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Inspired by the DCM degradation pathway of <font style="font-style: italic;">M. extorquens</font> DM4, our project is driven and refined by the dialogue between modelling simulations and experimental data. Bioremediation is optimised by expressing the DCM degrading system in host strains, along with microcompartments to accelerate the reaction and minimise toxic intermediates. Our biosensor is tuned to our characterisation and improvements of the catalytic efficiency of the system, while incorporation of the bacteria into novel diffusion-limiting biopolymeric beads ensures safe and rapid degradation.
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<br>
 +
This all-round modular design and scalability make DCMation ideal for extension to the disposal of many harmful substances. Explore our wiki for more!
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University of Oxford’s first iGEM team presents: DCMation, a demonstration of a novel bioremediation system whose potential applications are limited only by the versatility of bacterial metabolism. OxiGEM are tackling environmental pollution by developing a user-friendly and scalable device for the detection & degradation of the hazardous yet indispensable solvent dichloromethane (DCM). <br><br>
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      </div>
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      </div>
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Building on the DCM-degradation pathway of M. extorquens DM4, our project is driven and refined by the <font style="font-weight:700;">dialogue</font> between modelling simulations and experimental data. We are expressing intracellular microcompartments to accelerate and contain the reaction, preventing accumulation of toxic intermediates, while maximising the sensitivity and catalytic efficiency of the system through directed evolution. We are additionally constructing a synthetic fluorescent biosensor and incorporating our engineered bacteria into diffusion-limiting biopolymeric beads to ensure safety, efficiency and ease of use.<br><br>
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      <div id="menu1" style="position:absolute; display:none; colour:white; width:90%;height:92%;margin-left:5%;margin-right:5%;margin-top:2%;overflow-y:visible;overflow-x:visible;">
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The all-round modular design is ideal for extension to the disposal of many harmful substrates.
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<img src="https://static.igem.org/mediawiki/2014/7/78/OxigemBiosensor1.png" style="position:absolute; width:106%;margin-left:-3%;margin-top:-10%;"  />
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<a href="#"><img id="next1" src="https://static.igem.org/mediawiki/2014/7/73/OxigemNext.png" style="position:absolute;width:3%;margin-left:101.5%;margin-top:27%;"  /></a>
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<a href="https://2014.igem.org/Team:Oxford/biosensor"><img class="zero" src="https://static.igem.org/mediawiki/2014/a/af/Oxigem1.0.jpg" style="width:90%;margin-left:5%;margin-top:28%;" /></a>
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<a href="https://2014.igem.org/Team:Oxford/biosensor_characterisation"><img class="one" src="https://static.igem.org/mediawiki/2014/f/f8/Oxigem1.1.jpg" style="width:90%;margin-left:5%;margin-top:0.2%;" /></a>
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<a href="https://2014.igem.org/Team:Oxford/biosensor_construction"><img class="two" src="https://static.igem.org/mediawiki/2014/8/84/Oxigem1.2.jpg" style="width:90%;margin-left:5%;margin-top:0.2%;" /></a>
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<a href="https://2014.igem.org/Team:Oxford/biosensor_optimisation"><img class="three" src="https://static.igem.org/mediawiki/2014/d/df/Oxigem1.3.jpg" style="width:90%;margin-left:5%;margin-top:0.2%;" /></a>
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<img src="https://static.igem.org/mediawiki/2014/e/e8/OxigemBioremediation1.png" style="position:absolute; width:106%;margin-left:-3%;margin-top:-10%;"  />
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<a href="#"><img id="next2" src="https://static.igem.org/mediawiki/2014/7/73/OxigemNext.png" style="position:absolute;width:3%;margin-left:101.5%;margin-top:27%;"  /></a>
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<a href="https://2014.igem.org/Team:Oxford/bioremediation"><img class="one" src="https://static.igem.org/mediawiki/2014/d/d3/Oxigem2.0.jpg" style="width:90%;margin-left:5%;margin-top:28%;" /></a>
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<a href="https://2014.igem.org/Team:Oxford/how_much_can_we_degrade?"><img class="two" src="https://static.igem.org/mediawiki/2014/2/21/Oxigem2.1.jpg" style="width:90%;margin-left:5%;margin-top:0.2%;" /></a>
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<a href="https://2014.igem.org/Team:Oxford/what_are_microcompartments?"><img class="two" src="https://static.igem.org/mediawiki/2014/b/bb/Oxigem2.2.jpg" style="width:90%;margin-left:5%;margin-top:0.2%;" /></a>
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<img src="https://static.igem.org/mediawiki/2014/1/1b/OxigemRealisation1.png" style="position:absolute; width:106%;margin-left:-3%;margin-top:-10%;"  />
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<a href="#"><img id="back2" src="https://static.igem.org/mediawiki/2014/0/0c/OxigemBack.png" style="position:absolute;width:3%;margin-left:-4.5%;margin-top:27%;"  /></a>
 +
<a href="https://2014.igem.org/Team:Oxford/biosensor_realisation"><img class="one" src="https://static.igem.org/mediawiki/2014/f/f6/Oxigem3.1.jpg" style="width:90%;margin-left:5%;margin-top:28%;" /></a>
 +
<a href="https://2014.igem.org/Team:Oxford/realisation_bioremediation"><img class="two" src="https://static.igem.org/mediawiki/2014/1/1b/Oxigem3.2.jpg" style="width:90%;margin-left:5%;margin-top:0.2%;" /></a>
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<a href="https://2014.igem.org/Team:Oxford/biopolymer_containment"><img class="three" src="https://static.igem.org/mediawiki/2014/9/91/Oxigem3.3.jpg" style="width:90%;margin-left:5%;margin-top:0.2%;" /></a>
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       <img id="down" src="https://static.igem.org/mediawiki/2014/1/1c/OxigemDown.png" style="position: absolute; width:65%; top: 0; left: 17%;">
       <img id="down" src="https://static.igem.org/mediawiki/2014/1/1c/OxigemDown.png" style="position: absolute; width:65%; top: 0; left: 17%;">
       <a href="#"><img id="split" src="https://static.igem.org/mediawiki/2014/2/21/OxigemBlank.png" onclick="showabstract()" style="position: absolute; width:24%; top:35%; left: 38%;" /></a>
       <a href="#"><img id="split" src="https://static.igem.org/mediawiki/2014/2/21/OxigemBlank.png" onclick="showabstract()" style="position: absolute; width:24%; top:35%; left: 38%;" /></a>
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       <div class="Heading" id="DCMationheading" style="top: 72.2%; left: 34.8%; width:11%;" onclick="DCMation()" onmouseover=
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       "DCMationbig(this)" onmouseout="DCMationnormal(this)" border="0"><a href="#"><img src=
       "https://static.igem.org/mediawiki/2014/2/22/OxigemDCMationheading.png" style="width: 100%;" ></a>
       "https://static.igem.org/mediawiki/2014/2/22/OxigemDCMationheading.png" style="width: 100%;" ></a>
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       </div>
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       "Achievementsnormal(this)" border="0"><a href="#"><img src=
       "Achievementsnormal(this)" border="0"><a href="#"><img src=
         "https://static.igem.org/mediawiki/2014/d/db/OxigemAchievements.png" style="width: 100%;"></a>
         "https://static.igem.org/mediawiki/2014/d/db/OxigemAchievements.png" style="width: 100%;"></a>
       </div>
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       <div class="Heading" id="Modellingheading" style="top: 57%; left: 21%; width:8.5%;" onmouseover="Modellingbig(this)" onmouseout=
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         <a href="#"><img src=
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         <a href="https://2014.igem.org/Team:Oxford/Modelling"><img src=
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         "https://static.igem.org/mediawiki/2014/c/cd/OxigemModelling.png" style="width: 100%;"></a>
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         <a href="https://2014.igem.org/Team:Oxford/policy_and_practices"><img src=
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       <div class="Heading" id="iGEMcommheading" style="top: 27%; left: 24.5%; width:8.8%;" onclick="iGEMcomm()" onmouseover="iGEMcommbig(this)" onmouseout=
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         <a href="#"><img src=
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         "https://static.igem.org/mediawiki/2014/6/66/OxigemIGEMcomm.png" style="width: 100%;"></a>
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       <div class="Heading" id="interlabheading" style="top: 27.5%; left: 68%; width:6.5%;" onclick="Interlab()" onmouseover="Interlabbig(this)" onmouseout=
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       <div class="Heading" id="Safetyheading" style="top: 15.3%; left: 40%; width:5%;" onmouseover="Safetybig(this)" onmouseout=
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           <img src="https://static.igem.org/mediawiki/2014/3/32/OxigemDcmationbg.png" style="width: 100%;">
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           <div class="Heading" style="top: 14.2%; left: 31%; width:38%;" onmouseover="Subheadingbig(this)" onmouseout=
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Latest revision as of 19:15, 2 June 2015

Retrieved from "http://2014.igem.org/Team:Oxford"