Team:NTU Taida/M5

From 2014.igem.org

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Then the curve fitting part will be the future work.
Then the curve fitting part will be the future work.
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===settings for standard figure:===
[[File:M1.jpg| 600px| thumb |center |  ]]
[[File:M1.jpg| 600px| thumb |center |  ]]
[[File:M2.jpg| 600px| thumb |center |  ]]
[[File:M2.jpg| 600px| thumb |center |  ]]
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[[File:M3.jpg| 600px| thumb |center |  ]]
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===Different comparisons among parameter adjustment===
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[[File:M4.jpg| 600px| thumb |center |  ]]
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[[File:M3.jpg| 600px| thumb |center |  (1).        adjustment of TE value :
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[[File:M5.jpg| 600px| thumb |center |  ]]
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Findings: TE value is the absolute rather than the relative value of rbs. Ajusting TE  value can alter the magnitude of concentration.  (comparison with standard)
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[[File:M6.jpg| 600px| thumb |center |  ]]
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]]
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[[File:M7.jpg| 600px| thumb |center |  ]]
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[[File:M4.jpg| 600px| thumb |center | (2). Adjustment of degration rate of mRNA and Protein :
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[[File:M8.jpg| 600px| thumb |center |  ]]
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Findings: Ajusting degration rate can alter the time scale. (comparison with standard)
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We illustrate the result by showing different combinations of adjusting degration rate of mRNA and protein.
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❖ altering protein degration rate]]
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[[File:M5.jpg| 600px| thumb |center |  ❖ altering mRNA degration rate]]
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[[File:M6.jpg| 600px| thumb |center |  ❖ altering both protein and mRNA degration rate]]
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[[File:M7.jpg| 600px| thumb |center | (3).        adjustment of promoter PfadBA strength: 
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Findings: Increasing PfadBA streingth  will  increase concentration of CI and decrease the concentration of GFP. (comparison with standard)
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  ]]
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[[File:M8.jpg| 600px| thumb |center | (4).        adjustment of  the concentration of ayl-CoA:
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Findings: decreasing the concentration of acyl-coA, the concentration of CI will decrease and GFP will increase. (comparison with standard)
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  ]]
[[File:M10.jpg| 600px| thumb |center | (5).        Overexpression of FadL and FadR :
[[File:M10.jpg| 600px| thumb |center | (5).        Overexpression of FadL and FadR :
Findings:  Thereotically, when we overexpression the FadL and FadR (under non-overexpression of acyl-coA), the concentration of CI must decrease due to the competition between acyl-coA and FadR.
Findings:  Thereotically, when we overexpression the FadL and FadR (under non-overexpression of acyl-coA), the concentration of CI must decrease due to the competition between acyl-coA and FadR.

Revision as of 03:41, 18 October 2014

NTU-Taida

Curve fitting

Besides the simulation of our system, data processing is needed for. Then the curve fitting part will be the future work.

settings for standard figure:

M1.jpg
M2.jpg

Different comparisons among parameter adjustment

(1). adjustment of TE value : Findings: TE value is the absolute rather than the relative value of rbs. Ajusting TE value can alter the magnitude of concentration. (comparison with standard)
(2). Adjustment of degration rate of mRNA and Protein : Findings: Ajusting degration rate can alter the time scale. (comparison with standard) We illustrate the result by showing different combinations of adjusting degration rate of mRNA and protein. ❖ altering protein degration rate
❖ altering mRNA degration rate
❖ altering both protein and mRNA degration rate
(3). adjustment of promoter PfadBA strength: Findings: Increasing PfadBA streingth will increase concentration of CI and decrease the concentration of GFP. (comparison with standard)
(4). adjustment of the concentration of ayl-CoA: Findings: decreasing the concentration of acyl-coA, the concentration of CI will decrease and GFP will increase. (comparison with standard)
(5). Overexpression of FadL and FadR : Findings: Thereotically, when we overexpression the FadL and FadR (under non-overexpression of acyl-coA), the concentration of CI must decrease due to the competition between acyl-coA and FadR. However, the result remain the same. We assume that it might be our limitation of curve fitting due to the lack of experimental data. And thus we aren’t able to use the whole differential functions(see the parameter setting and initial conditions above)