Result
Mlr A has significant degradation function to MC-LR, and at certain degree of MC-LR, the natural promoter will promote the expression of gene in engineering bacteria. This is a lucky accident. Before this, we have tried to use PP2A to monitor the concentration of MC-LR. But we found it was really hard for us to do the gene control in Prokaryotic cells. However, we are lucky to find the natural promoter of Mlr A which gave us a new approach to realize the goal of our project.
Blueprint
Until I write this paragraph, our team have found a promoter to detect the toxin and a enzyme to decompose it. The result show a good biological activity and I think it can be used in our daily life what I will discuss next. We can see that we already achieve our goal：detecting the density of MC-LR and release MlrA by itself. Our team call it “Automatization machine control”.
We can use this result in water cleansing by clearing MC-LR before people drink or use. According to report, MC-LR can destroy human’s liver, so what we do not only protect environment but make people healthier. Instead of E.coli, we use lactic acid bacteria as our engineering bacteria to obey the rule that working for people’s healthy.
Lower the danger level and make it more automate, we got it and will make it much better.
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