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May, 2014
- Data query and project theme select
- Experiment scheme design
- Codon optimization of MlrA gene by Lactococcus lactis codons）
- MlrA gene sequence design and split
- RFP-Mlr-GFP sequence design and split
June, 2014
- MlrA gene synthesis and sequence
- RFP-Mlr-GFP gene synthesis and sequence
July, 2014
- MlrA gene expression and detection
- RFP-Mlr-GFP gene expression and detection
- Screening of microcystin LR sensitive promoter
August, 2014
- construction of the recombinant vector pMG-mlr
- Recombinant vector was transformed into Escherichia coli
- GFP-mlrA expression by mlr promoter and detection in Escherichia coli
- Detection of microcystins LR degradation ability in water by Recombinant Escherichia coli
September, 2014
- Recombinant vector was transformed into Lactococcus lactis
- GFP-mlrA expression by mlr promoter and detection in Lactococcus lactis
- Detection of microcystins LR degradation ability in water by Recombinant Lactococcus lactis
October, 2014
- Detection of microcystins LR degradation ability in the natural environment by Recombinant Escherichia coli
- Detection of microcystins LR degradation ability in the natural environment by Recombinant Lactococcus lactis
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