Team:Hong Kong HKUST/wetlab/notebook

From 2014.igem.org

(Difference between revisions)
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<h3>Notebook</h3>
<h3>Notebook</h3>
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<tr class= "content_row">
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<p>
 +
Throughout the course of our project, we have continuously recorded our daily work, such as wetlab activities and
 +
research findings. The notebook for Pneuumonsensor and Riboregulator are displayed separately.
 +
 +
</p>
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<div id="satu" align="center"> <h2>June 2013</h2>
<div id="satu" align="center"> <h2>June 2013</h2>
    
    
       <div>
       <div>
-
       <a href="#" class="head">Week 4</a>
+
       <a href="#" class="head">Week 1</a>
       <div class="content" align="left" style="display: none;">
       <div class="content" align="left" style="display: none;">
-
<br><b>June 24</b><br>
+
<br><b>June xx</b> <br>
-
Wet lab <br>
+
Wet lab<br>
-
·      Inoculation of pBlueScript KS(+) for training <br>
+
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
-
·      Autoclave basic materials <br>
+
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
-
·      Preparing LB <br>
+
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
-
·      LB-Ampicillin plates poured
+
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
-
Dry lab <br>
+
<br><b>June xx</b> <br>
-
Protocols review <br>
+
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
-
<br><b>
 
-
June 25 </b><br>
 
-
Wet lab <br>
 
-
·  Plasmid extraction of pBlueScript KS(+)  <br>
 
-
·      LB-Chloramphenicol plates poured <br>
 
-
Dry lab <br>
 
-
·      Primers design for FABP1 promoter, PPAR-alpha promoter, GRP78 promoter <br>
 
-
·      Informal meeting <br>
 
-
<br><b>
+
      </div>
-
June 26 </b><br>
+
    </div>
-
Wet lab <br>
+
<div>
-
·      Extraction of gDNA from HepG2 Cells for FABP1 promoter and PPAR-alpha promoter <br>
+
      <a href="#" class="head">Week 2</a>
-
·      Transformation of pEGFP-N1 and <a href="http://parts.igem.org/Part:BBa_K817002">BBa_K817002</a> (P<i>fadBA</i>) <br>
+
      <div class="content" align="left" style="display: none;">
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
-
<br><b>
+
      </div>
-
June 27 </b><br>
+
    </div>
-
Wet lab <br>
+
<div>
-
·      Inoculation of pEGFP-N1 and BBa_K817002 (P<i>fadBA</i>) for plasmid extraction <br>
+
      <a href="#" class="head">Week 3</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
-
<br><b>
 
-
June 28</b> <br>
 
-
Wet lab <br>
 
-
·      Extraction of pEGFP-N1 and BBa_K817002 (P<i>fadBA</i>)  plasmids by miniprep <br>
 
-
·      Restriction of pEGFP-N1 Restriction digestion by Ase1 and BamH1 for PPAR-alpha promoter; Ase1 and Xho1 for GRP78, Xba1 and BamH1 for FABP1 promoter, Pst1 and Not1; followed by gel check, extraction and purification <br>
 
-
·      Restriction of c(P<i>fadBA</i>) by EcoR1 and Pst1 <br>
 
-
·      Ran 0.8% gel for pEGFP-N1 and 2% gel for BBa_K817002 (P<i>fadBA</i>) <br>
 
-
<br><b>
+
      </div>
-
June 30 </b><br>
+
    </div>
-
Dry lab <br>
+
<div>
-
Experiment planning and protocols revision for next week work <br><br>
+
      <a href="#" class="head">Week 4</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 
 +
 
 +
      </div>
 +
    </div>
 +
<div>
 +
      <a href="#" class="head">Week 5</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
       </div>
       </div>
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  </div>
</div>
</div>
 +
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<div id="satu" align="center"> <h2><center>July 2013</center></h2>
<div id="satu" align="center"> <h2><center>July 2013</center></h2>
    
    
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   </div>
  </div>
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 +
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 +
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 +
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 +
<div id="satu" align="center"> <h2>August 2013</h2>
<div id="satu" align="center"> <h2>August 2013</h2>
    
    
<div>
<div>
 +
      <a href="#" class="head">Week 1</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
 +
      </div>
 +
    </div>
 +
<div>
 +
      <a href="#" class="head">Week 2</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
      </div>
 +
    </div>
 +
<div>
 +
      <a href="#" class="head">Week 3</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
 +
      </div>
 +
    </div>
 +
<div>
 +
      <a href="#" class="head">Week 4</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
 +
      </div>
 +
    </div>
 +
<div>
 +
      <a href="#" class="head">Week 5</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
 +
      </div>
 +
    </div>
 +
 +
      </div>
 +
    </div>
 +
</div>
 +
<!---- SSSSSSSS  EEEEEEEE  PPPPPPPP  TTTTTTTTTTTT  EEEEEEEE  M        M  BBBBBBBB    EEEEEEEEE  RRRRRRRR    2 0 1 3-------->
 +
<!---- S          E          P      P      T        E          MM      MM  B      B  E          R    R 2 0 1 3-------->
 +
<!---- S          E          P      P        T        E          M M    M M  B      B  E          R    R 2 0 1 3-------->
 +
<!---- S          E          P      P        T        E          M  M  M  M  B      B  E          R  R 2 0 1 3-------->
 +
<!----     S          E          P      P        T        E          M  M M  M  B      B  E          R    R 2 0 1 3-------->
 +
<!----      SSSSSSSS  EEEEEEEE  PPPPPPPP        T        EEEEEEEE  M    M    M  BBBBBBBB    EEEEEEEEE  RRRRRRRR 2 0 1 3-------->
 +
<!----            S  E          P              T        E          M        M  B      B  E          R  R      2 0 1 3-------->
 +
<!----   S  E          P              T        E          M        M  B      B  E          R    R  2 0 1 3-------->
 +
<!----   S  E          P              T        E          M        M  B      B  E          R    R 2 0 1 3-------->
 +
<!----   S  E          p              T        E          M        M  B      B  E          R      R 2 0 1 3-------->
 +
<!---- SSSSSSSS  EEEEEEEE  P              T        EEEEEEEE  M        M  BBBBBBBB    EEEEEEEEE  R  R 2 0 1 3-------->
 +
 +
<div id="satu" align="center"> <h2>September 2013</h2>
 +
 
 +
<div>
       <a href="#" class="head">Week 1</a>
       <a href="#" class="head">Week 1</a>
       <div class="content" align="left" style="display: none;">
       <div class="content" align="left" style="display: none;">
Line 419: Line 743:
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
 +
      </div>
 +
    </div>
 +
<div>
 +
      <a href="#" class="head">Week 2</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
      </div>
 +
    </div>
 +
<div>
 +
      <a href="#" class="head">Week 3</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
 +
      </div>
 +
    </div>
 +
<div>
 +
      <a href="#" class="head">Week 4</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
 +
      </div>
 +
    </div>
 +
<div>
 +
      <a href="#" class="head">Week 5</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
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Revision as of 14:06, 2 October 2014

Notebook

Throughout the course of our project, we have continuously recorded our daily work, such as wetlab activities and research findings. The notebook for Pneuumonsensor and Riboregulator are displayed separately.

June 2013

Week 1
Week 2
Week 3
Week 4
Week 5

July 2013

Week 1
Week 2
Week 3
Week 4
Week 5

August 2013

Week 1
Week 2
Week 3
Week 4
Week 5

September 2013

Week 1
Week 2
Week 3
Week 4
Week 5

Home

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Retrieved from "http://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/notebook"