Team:HokkaidoU Japan/Notebook/asB0034/asB0034-construction
From 2014.igem.org
(Difference between revisions)
(12 intermediate revisions not shown) | |||
Line 5: | Line 5: | ||
{{Team:HokkaidoU_Japan/LargeDropDown/CSS}} | {{Team:HokkaidoU_Japan/LargeDropDown/CSS}} | ||
<html> | <html> | ||
- | < | + | <link href='http://fonts.googleapis.com/css?family=Merriweather' rel='stylesheet' type='text/css'> |
<div id="background"> | <div id="background"> | ||
<!--begin header--> | <!--begin header--> | ||
Line 13: | Line 13: | ||
<div id="header-bottom"> | <div id="header-bottom"> | ||
<div id="header-bottom-contents"> | <div id="header-bottom-contents"> | ||
- | + | <div class="header-title"> | |
+ | <div class="header-title-top"> | ||
+ | Notebook | ||
+ | </div> | ||
+ | <div class="header-title-bottom"> | ||
+ | Lab Documents | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="header-picture"> | ||
+ | <img src="https://static.igem.org/mediawiki/2014/4/44/HokkaidoU_Header_ki.png"> | ||
+ | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
Line 29: | Line 39: | ||
<div class="ldd_submenu" style="left:-415px; width:970px;"> | <div class="ldd_submenu" style="left:-415px; width:970px;"> | ||
<ul style="margin-left:20px;"> | <ul style="margin-left:20px;"> | ||
- | <li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Overview/ | + | <li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Overview/Background">Overview</a></li> |
- | + | ||
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Overview/Background">Background</a></li> | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Overview/Background">Background</a></li> | ||
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Overview/Achievements">Achievements</a></li> | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Overview/Achievements">Achievements</a></li> | ||
Line 51: | Line 60: | ||
<ul> | <ul> | ||
<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Length">Length Variation</a></li> | <li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Length">Length Variation</a></li> | ||
- | <li class="ldd_contents"><a href="">Overview</a></li> | + | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Length">Overview</a></li> |
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Length#Method">Method</a></li> | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Length#Method">Method</a></li> | ||
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Length#Results">Results</a></li> | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Length#Results">Results</a></li> | ||
Line 73: | Line 82: | ||
</ul> | </ul> | ||
<ul> | <ul> | ||
- | <li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey">High | + | <li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey">High School</a></li> |
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey#Education">Education</a></li> | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey#Education">Education</a></li> | ||
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey#Survey">Survey</a></li> | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey#Survey">Survey</a></li> | ||
Line 80: | Line 89: | ||
<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li> | <li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li> | ||
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li> | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li> | ||
- | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion# | + | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Evaluation">Evaluation</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
Line 129: | Line 138: | ||
</ul> | </ul> | ||
<div class="step-recipe"> | <div class="step-recipe"> | ||
- | + | asB0034_F Tm:68.7℃ asB0034_R Tm:68.7℃ | |
</div> | </div> | ||
</div> | </div> | ||
Line 234: | Line 243: | ||
</ul> | </ul> | ||
<div class="step-recipe"> | <div class="step-recipe"> | ||
- | Ptrc-stem-asB0034-stem | + | Ptrc-stem-asB0034-stem on pHN1257 |
</div> | </div> | ||
+ | <div class="step-recipe"> | ||
+ | 5.0µL DNA to DH5α | ||
+ | |||
</div> | </div> | ||
</div> | </div> | ||
Line 255: | Line 267: | ||
</ul> | </ul> | ||
<div class="step-recipe"> | <div class="step-recipe"> | ||
- | Ptrc-stem-asB0034-stem(pHN1257) pHN1257_up_F Tm:60.1 asB0034-R Tm:68.7 | + | Ptrc-stem-asB0034-stem(on pHN1257) pHN1257_up_F Tm:60.1℃ & asB0034-R Tm:68.7℃ |
</div> | </div> | ||
</div> | </div> | ||
Line 276: | Line 288: | ||
</ul> | </ul> | ||
<div class="step-recipe"> | <div class="step-recipe"> | ||
- | Ptrc-stem-asB0034-stem | + | Ptrc-stem-asB0034-stem(on pHN1257) |
</div> | </div> | ||
</div> | </div> | ||
Line 284: | Line 296: | ||
<div class="step-tile"> | <div class="step-tile"> | ||
<h2 class="step-title"> | <h2 class="step-title"> | ||
- | + | Mini-Prep | |
</h2> | </h2> | ||
</div> | </div> | ||
Line 297: | Line 309: | ||
</ul> | </ul> | ||
<div class="step-recipe"> | <div class="step-recipe"> | ||
- | Ptrc-stem-asB0034-stem | + | Ptrc-stem-asB0034-stem(on pHN1257) |
</div> | </div> | ||
</div> | </div> | ||
Line 305: | Line 317: | ||
<div class="step-tile"> | <div class="step-tile"> | ||
<h2 class="step-title"> | <h2 class="step-title"> | ||
- | Transformation | + | Double Transformation |
</h2> | </h2> | ||
</div> | </div> | ||
Line 318: | Line 330: | ||
</ul> | </ul> | ||
<div class="step-recipe"> | <div class="step-recipe"> | ||
- | Ptrc-stem-asB0034-stem on pHN1257& | + | Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(on pSB6A1) |
+ | </div> | ||
+ | <div class="step-recipe"> | ||
+ | each of 2.5µL DNA to DH5α | ||
</div> | </div> | ||
</div> | </div> | ||
Line 335: | Line 350: | ||
<div id="footer-content"> | <div id="footer-content"> | ||
<div id="footer-logo"> | <div id="footer-logo"> | ||
- | <a href="http://igemhokkaidou.com | + | <a href="http://igemhokkaidou.wordpress.com"><img style="height:150px;position:relative;" src="https://static.igem.org/mediawiki/2014/3/39/HokkaidoU_logo_transparent.png"></a> |
</div> | </div> | ||
<div id="footer-twitter"> | <div id="footer-twitter"> |
Latest revision as of 15:24, 9 September 2015
Notebook
Lab Documents
asB0034 Construction
-
Start
-
Primer Annealing
asB0034_F Tm:68.7℃ asB0034_R Tm:68.7℃ -
Digestion
Cut pHN1257 with NcoI, XhoI(using 10×cut smart) -
Gel extraction
digested pHN1257 -
Ethanol Precipetation
digested pHN1257 -
Ligation
Ligate pHN1257 with asB0034 -
Transformation
Ptrc-stem-asB0034-stem on pHN12575.0µL DNA to DH5αColony PCR
Ptrc-stem-asB0034-stem(on pHN1257) pHN1257_up_F Tm:60.1℃ & asB0034-R Tm:68.7℃Culture
Ptrc-stem-asB0034-stem(on pHN1257)Mini-Prep
Ptrc-stem-asB0034-stem(on pHN1257)Double Transformation
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(on pSB6A1)each of 2.5µL DNA to DH5αComplete!