Team:HokkaidoU Japan/Notebook/asB0034/asB0034,32-1C3-construction

(Difference between revisions)

Revision as of 02:21, 18 October 2014

Notebook

Lab Documents

Start
Digestion
- Mon Sep 29
Cut vector with EcoRI, PstI (using 10×H buffer) Cut insert with EcoRI, PstI (using 10×M buffer)
Digestion
- Tue Sep 30
Cut vector with EcoRI, PstI (using 10×H buffer)
Gel extraction
- Tue Sep 30
digested vector insert
Ethanol Precipitation
- Tue Sep 30
digested vector insert
Ligation
- Tue Sep 30
Ligate vector with insert
Transformation
- Tue Sep 30
ligation products 5.0µL to DH5α
Colony PCR
- Wed Oct 1
R0010-stem-asB0034-stem-B0015(on pSB1C3) 100up_F Tm:62.8℃ & asB0034_R Tm:68.7℃ R0010-stem-asB0032-stem-B0015(on pSB1C3) 100up_F Tm:62.8℃ & asB0032_R Tm:70.8℃
Culture
- Wed Oct 1
R0010-stem-asB0034-stem-B0015(on pSB1C3) R0010-stem-asB0032-stem-B0015(on pSB1C3)
Mini-Prep
- Wed Oct 1
R0010-stem-asB0034-stem-B0015(on pSB1C3) R0010-stem-asB0032-stem-B0015(on pSB1C3)
Sequencing
- Wed Oct 1
R0010-stem-asB0034-stem-B0015(on pSB1C3) with 100up_F Tm:62.8℃ & 200dn:66.6℃ R0010-stem-asB0032-stem-B0015(on pSB1C3) with 100up_F Tm:62.6℃ 200dn_R Tm:66.6℃
Complete!

@@ Line 266: / Line 266: @@
 	      </ul>
 	      <div class="step-recipe">
-R0010-stem-asB0034-stem-B0015(on pSB1C3) 100up_F	Tm:62.8&#8451; & asB0034_R	Tm:68.7&#8451; R0010-stem-asB0032-stem-B0015(on pSB1C3) 100up_F	Tm:74.5&#8451; & asB0032_R	Tm:70.8&#8451;
+R0010-stem-asB0034-stem-B0015(on pSB1C3) 100up_F	Tm:62.8&#8451; & asB0034_R	Tm:68.7&#8451; R0010-stem-asB0032-stem-B0015(on pSB1C3) 100up_F	Tm:62.8&#8451; & asB0032_R	Tm:70.8&#8451;
 </div>
 	    </div>
@@ Line 329: / Line 329: @@
 	      </ul>
 	      <div class="step-recipe">
-R0010-stem-asB0034-stem-B0015(on pSB1C3) 100up 200dn R0010-stem-asB0032-stem-B0015(on pSB1C3) 100up 200dn
+R0010-stem-asB0034-stem-B0015(on pSB1C3) with 100up_F Tm:62.8&#8451; & 200dn:66.6&#8451; R0010-stem-asB0032-stem-B0015(on pSB1C3) with 100up_F Tm:62.6&#8451; 200dn_R Tm:66.6&#8451;
 </div>
 	    </div>