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Team:HokkaidoU Japan/Notebook/asB0034/Ptet B0034 32 asB0034 assay
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Latest revision as of 15:32, 9 September 2015
Notebook
Lab Documents
Ptet-B0034,B0032,-mRFP-dT
Ptet-B0032-mRFP-dT&asB0034Assay
-
Start
-
Preculture
R0040-B0034-E1010-B0015 (on pSB6A1)R0040-B0032-E1010-B0015 (on pSB6A1)Ptrc-stem-asB0034-stem(on pHN1257)R0040-B0032-E1010-B0015 (on pSB6A1)Turbidity was controlled 0.1 -
Culture
M9ZB 2 mL IPTG 20 µ or not for 9 hours,11 hours, 13hours -
Assay
fluoresence imageing 514 nm 610 filter -
Complete!
